Abstract
Hundreds of RNA-binding proteins are known, but the biological functions are known for only a few of them. They regulate various aspects of RNA processing or biogenesis such as splicing, polyadenylation, and translation. Here I describe a bioinformatics approach that we developed to identify potential new mRNA target(s) of the Drosophila master sex-switch protein Sex-lethal (SXL) by combining computational analysis with genetic and biochemical investigation. This approach could be used to identify new RNA–protein interactions during oogenesis in the female germline and should be applicable to numerous other posttranscriptional regulatory events.
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Acknowledgements
I thank Andrew Rahn and Mark Robida for their notes consulted for materials and methods and the American Cancer Society and the Butcher Foundation for grants to RS that supported this work.
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Singh, R. (2015). Bioinformatics Analysis to Identify RNA–Protein Interactions in Oogenesis. In: Bratu, D., McNeil, G. (eds) Drosophila Oogenesis. Methods in Molecular Biology, vol 1328. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2851-4_17
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DOI: https://doi.org/10.1007/978-1-4939-2851-4_17
Publisher Name: Humana Press, New York, NY
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