Abstract
Studying the mechanisms of entry of cell-penetrating peptides (CPPs) requires reliable methods to measure their cellular uptake efficiency, monitor their metabolic stability, and identify their intracellular localization. We describe here a protocol based on the direct detection of peptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which allows the absolute quantification of the intact internalized species and the analysis of their intracellular degradation. This protocol can be easily applied to the simultaneous quantification of different species, for example mixtures of CPPs.
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Acknowledgments
We thank Dr. G. Chassaing, Dr. G. Bolbach, Dr. C.-Y. Jiao, Dr. S. Aubry, Dr. B. Aussedat, Dr. D. Delaroche, S. Bode, and M. Amoura for their contribution to the development of the CPP quantification protocol.
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Sagan, S., Bechara, C., Burlina, F. (2015). Study of CPP Mechanisms by Mass Spectrometry. In: Langel, Ü. (eds) Cell-Penetrating Peptides. Methods in Molecular Biology, vol 1324. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2806-4_7
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DOI: https://doi.org/10.1007/978-1-4939-2806-4_7
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2805-7
Online ISBN: 978-1-4939-2806-4
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