Abstract
Self-assembling RNA scaffold is designed to co-localize enzymes in engineered biological pathways through interactions between scaffold’s protein docking domains and their affinity protein–enzyme fusions, in vivo. Here we introduced a noncoding RNA structure theophylline aptamer that respond to theophylline ligand in order to modulate RNA scaffold and regulate multistep enzymatic activities. We described the specifically designed RNA scaffold increased the fluorescent intensity in a splitting GFP assay by 2.25-fold, and also observed a 1.43-fold increase in multi-enzymatic efficiency in IAA synthesis pathway.
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ZJU_China Team (iGEM 2012)., Chen, M. (2015). RNA Scaffold: Designed to Co-localize Enzymes. In: Ponchon, L. (eds) RNA Scaffolds. Methods in Molecular Biology, vol 1316. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2730-2_9
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DOI: https://doi.org/10.1007/978-1-4939-2730-2_9
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2729-6
Online ISBN: 978-1-4939-2730-2
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