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Strategic RNA Silencing for Plant Viral Resistance

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Somatic Genome Manipulation

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Abstract

Viral diseases inflict substantial economic losses to major crops by reducing yield and compromising quality. RNA silencing using, e.g. self-complementary hairpin RNA (hpRNA) or artificial microRNA (amiRNA), is an effective method to produce plants that are resistant to specific virus(es). By targeting highly conserved viral sequences or several virus genes simultaneously using chimeric constructs, this method can counter multiple viruses and minimize any loss of viral resistance resulting from viral mutation. Due to public concerns about transgenic plant safety, a non-transgenic RNA silencing approach was used to directly deliver hpRNA into plant tissues to induce plant resistance to viruses.

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Authors and Affiliations

  1. Forestry and Environmental Management, University of New Brunswick, E3B 6C2, Fredericton, NB, Canada

    Dr. Jin Ma

  2. Potato Research Center, Agriculture and Agri-Food Canada, P.O. Box 20280, E3B 4Z7, Fredericton, NB, Canada

    Dr. Jin Ma

  3. State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Tai’an, People’s Republic of China

    Dr. Changxiang Zhu & Prof. Fujiang Wen

  4. Canadian Food Inspection Agency, Charlottetown Laboratory, 93 Mount Edward Road, C1A 5T1, Charlottetown, PE, Canada

    Dr. Huimin Xu

  5. Potato Research Center, Agriculture and Agri-Food Canada, 850 Lincoln Road, P.O. Box 20180, E3B 4Z7, Fredericton, NB, Canada

    Dr. Xiu-Qing Li

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  1. Dr. Jin Ma
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  2. Dr. Changxiang Zhu
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  3. Prof. Fujiang Wen
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  4. Dr. Huimin Xu
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  5. Dr. Xiu-Qing Li
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Correspondence to Changxiang Zhu or Xiu-Qing Li .

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Editors and Affiliations

  1. Potato Research Centre, Agriculture and Agri-Food Canada, Government of Canada, Fredericton, New Brunswick, Canada

    Xiu-Qing Li

  2. Plant Science Department, McGill University, Sainte-Anne-de-Bellevue, Québec, Canada

    Danielle J. Donnelly

  3. Department of Biomedicine, Aarhus University, Herning, Denmark

    Thomas G. Jensen

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Ma, J., Zhu, C., Wen, F., Xu, H., Li, XQ. (2015). Strategic RNA Silencing for Plant Viral Resistance. In: Li, XQ., Donnelly, D., Jensen, T. (eds) Somatic Genome Manipulation. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2389-2_11

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