Abstract
Functional proteins have been found in infant milk formula as supplements, added by an increasing number of manufacturers. Their supplementations are expected to be controlled and monitored. Here, we describe a microchip-integrated CE method for the determination of these low levels of functional proteins in a protein-rich sample matrix. On-chip isoelectric focusing (IEF) is used to separate high-abundance proteins from low-abundance proteins instead of using some complicated time-consuming protein purification process. After that, transient isotachophoresis hyphenated capillary zone electrophoresis (t-ITP-CZE) can preconcentrate, separate, and analyze transferred functional proteins in the embedded capillary under UV detection.
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References
Fox PF, McSweeney PLH (1998) Milk proteins. In: Fox PF, McSweeney PLH (eds) Dairy chemistry and biochemistry. Blackie Academic, London, pp 146–238
Lien EL (2003) Infant formulas with increased concentrations of alpha-lactalbumin. Am J Clin Nutr 77:1555S–1558S
GB 14880-2012, Food safety national standards for the usage of nutrition enrichment, China
Palmano KP, Elgar DF (2002) Detection and quantitation of lactoferrin in bovine whey samples by reversed-phase high-performance liquid chromatography on polystyrene–divinylbenzene. J Chromatogr A 947:307–311
Hurley IP, Coleman RC, Ireland HE et al (2006) Use of sandwich IgG ELISA for the detection and quantification of adulteration of milk and soft cheese. Int Dairy J 16:805–812
Billakanti JM, Fee CJ, Lane FR et al (2010) Simultaneous, quantitative detection of five whey proteins in multiple samples by surface plasmon resonance. Int Dairy J 20:96–105
Yamada M, Murakami K, Wallingford JC et al (2002) Identification of low-abundance proteins of bovine colostral and mature milk using two-dimensional electrophoresis followed by microsequencing and mass spectrometry. Electrophoresis 23:1153–1160
Dalgleish DG (1990) Denaturation and aggregation of serum proteins and caseins in heated milk. J Agric Food Chem 38:1995–1999
Riechel P, Weiss T, Ulber R et al (1998) Analysis of bovine lactoferrin in whey using capillary electrophoresis (CE) and micellar electrokinetic chromatography (MEKC). Adv Exp Med Biol 443:33–39
Wu RG, Wang ZP, Zhao WF et al (2013) Multi-dimension microchip-capillary electrophoresis device for determination of functional proteins in infant milk formula. J Chromatogr A 1304:220–226
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© 2015 Springer Science+Business Media, New York
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Wu, R., Wang, Z., Fung, Y.S. (2015). Multidimensional Microchip-Capillary Electrophoresis Device for Determination of Functional Proteins in Infant Milk Formula. In: Van Schepdael, A. (eds) Microchip Capillary Electrophoresis Protocols. Methods in Molecular Biology, vol 1274. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2353-3_10
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DOI: https://doi.org/10.1007/978-1-4939-2353-3_10
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2352-6
Online ISBN: 978-1-4939-2353-3
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