Abstract
Mitochondria play a key role in various modes of cell death. Analysis of mitochondrial dysfunction and the release of proteins from the intermembrane space of mitochondria represent essential tools in cell death investigation. Here we describe how to evaluate release of intermembrane space proteins during apoptosis, alterations in the mitochondrial membrane potential, and oxygen consumption in apoptotic cells.
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Acknowledgments
The work was supported by grants from the Swedish Childhood Cancer Foundation, the Swedish Research Council, the Swedish and the Stockholm Cancer Societies, and the Russian Foundation for Basic Research (grant 14-04-00963 А).
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Gogvadze, V., Orrenius, S., Zhivotovsky, B. (2015). Analysis of Mitochondrial Dysfunction During Cell Death. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1264. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2257-4_33
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DOI: https://doi.org/10.1007/978-1-4939-2257-4_33
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