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Stem Cells, Neural Progenitors, and Engineered Stem Cells

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Neuronal Cell Death

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1254))

Abstract

Human pluripotent stem cells (hPSCs ) have the unique potential to form every cell type in the body. This potential provides opportunities for generating human progenitors and other differentiated cell types for understanding human development and for use in cell type-specific therapies . Equally important is the ability to engineer stem cells and their derived progenitors to mimic specific disease models. This chapter will focus on the propagation and characterization of human neural progenitors (hNPs ) derived from hPSCs with a particular focus on engineering hNPs to generate in vitro disease models for human neuro-mitochondrial disorders . We will discuss the methodologies for culturing and characterizing hPSCs and hNPs; and protocols for engineering hNPs by using a novel mitochondrial transfection technology.

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References

  1. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al (1998) Embryonic stem cell lines derived from human blastocysts. Science 282:1145–1147

    Article  CAS  PubMed  Google Scholar 

  2. Yu J, Vodyanik MA, Smuga-Otto K et al (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318:1917–1920

    Article  CAS  PubMed  Google Scholar 

  3. Takahashi K, Tanabe K, Ohnuki M et al (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861–872

    Article  CAS  PubMed  Google Scholar 

  4. Shin S, Mitalipova M, Noggle S et al (2006) Long-term proliferation of human embryonic stem cell-derived neuroepithelial cells using defined adherent culture conditions. Stem Cells 24:125–138

    Article  PubMed  Google Scholar 

  5. Dhara SK, Hasneen K, Machacek DW et al (2008) Human neural progenitor cells derived from embryonic stem cells in feeder-free cultures. Differentiation 76:454–464

    Article  CAS  PubMed  Google Scholar 

  6. Shin S, Dalton S, Stice SL (2005) Human motor neuron differentiation from human embryonic stem cells. Stem Cells Dev 14:266–269

    Article  CAS  PubMed  Google Scholar 

  7. Wilczynska KM, Singh SK, Adams B et al (2009) Nuclear factor I isoforms regulate gene expression during the differentiation of human neural progenitors to astrocytes. Stem Cells 27:1173–1181

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Iyer S, Alsayegh K, Abraham S et al (2009) Stem cell-based models and therapies for neurodegenerative diseases. Crit Rev Biomed Eng 37:321–353

    Article  PubMed  Google Scholar 

  9. Wilson PG, Cherry JJ, Schwamberger S et al (2007) An SMA project report: neural cell-based assays derived from human embryonic stem cells. Stem Cells Dev 16:1027–1041

    Article  CAS  PubMed  Google Scholar 

  10. Khan SM, Bennett JP Jr (2004) Development of mitochondrial gene therapy. J Bioenerg Biomembr 36:387–393

    Article  CAS  PubMed  Google Scholar 

  11. Iyer S, Thomas RR, Portell FR et al (2009) Recombinant mitochondrial transcription factor A with N-terminal mitochondrial transduction domain increases respiration and mitochondrial gene expression. Mitochondrion 9:196–203

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  12. Iyer S, Bergquist K, Young K et al (2012) Mitochondrial gene therapy improves respiration, biogenesis, and transcription in G11778A Leber’s hereditary optic neuropathy and T8993G Leigh’s syndrome cells. Hum Gene Ther 23:647–657

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Iyer S, Xiao E, Alsayegh K et al (2012) Mitochondrial gene replacement in human pluripotent stem cell-derived neural progenitors. Gene Ther 19:469–475

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported in part by start-up funds provided to Dr. Iyer by the Center for the Study of Biological Complexity at Virginia Commonwealth University and a grant from the Presidential Research Innovation Program (PRIP) at Virginia Commonwealth University. The authors would also like to thank Gencia Corporation, Charlottesville for provision of the recombinant human TFAM . Mention of vendor names is for information only and does not imply endorsement.

Author information

Authors and Affiliations

  1. Department of Chemical and Life Science Engineering, Virginia Commonwealth University, 601 West Main Street, Room 410, Richmond, VA, 23284-3028, USA

    Raj R. Rao & Shilpa Iyer

Authors
  1. Raj R. Rao
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  2. Shilpa Iyer
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Corresponding author

Correspondence to Shilpa Iyer .

Editor information

Editors and Affiliations

  1. Dept. of Veterinary Sciences, University of Torino, Grugliasco, Torino, Italy

    Laura Lossi

  2. Dept. of Veterinary Sciences, University of Torino, Grugliasco, Torino, Italy

    Adalberto Merighi

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Rao, R.R., Iyer, S. (2015). Stem Cells, Neural Progenitors, and Engineered Stem Cells. In: Lossi, L., Merighi, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 1254. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2152-2_19

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  • DOI: https://doi.org/10.1007/978-1-4939-2152-2_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2151-5

  • Online ISBN: 978-1-4939-2152-2

  • eBook Packages: Springer Protocols

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