Abstract
Ticks are blood-feeding arthropod ectoparasites of wild and domestic animals that transmit disease-causing pathogens to humans and animals worldwide and a good model for the characterization of tick-host-pathogen interactions. Tick-host-pathogen interactions consist of dynamic processes involving genetic traits of hosts, pathogens, and ticks that mediate their development and survival. Proteomics provides information on the protein content of cells and tissues that may differ from results at the transcriptomics level and may be relevant for basic biological studies and vaccine antigen discovery. In this chapter, we describe various methods for protein extraction and for proteomics analysis in ticks based on one-dimensional gel electrophoresis to characterize tick-host-pathogen interactions. Particularly relevant for this characterization is the use of blood-fed ticks. Therefore, we put special emphasis on working with replete ticks collected after feeding on vertebrate hosts.
Key words
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
de la Fuente J, Estrada-Peña A, Venzal JM et al (2008) Overview: Ticks as vectors of pathogens that cause disease in humans and animals. Front Biosci 13:6938–6946
Villar M, Popara M, Bonzón-Kulichenko E et al (2012) Characterization of the tick-pathogen interface by quantitative proteomics. Ticks Tick Borne Dis 3:154–158
de la Fuente J, Kocan KM, Blouin EF et al (2010) Functional genomics and evolution of tick-Anaplasma interactions and vaccine development. Vet Parasitol 167:175–186
Galindo RC, de la Fuente J (2012) Transcriptomics Data integration reveals Jak-STAT as a common pathway affected by pathogenic intracellular bacteria in natural reservoir hosts. J Proteomics Bioinform 5:108–115
de la Fuente J, Almazán C, Canales M et al (2007) A ten-year review of commercial vaccine performance for control of tick infestations on cattle. Anim Health Res Rev 8:23–28
de la Fuente J (2012) Vaccines for vector control: exciting possibilities for the future. Vet J 194:139–140
Villar M, Torina A, Nuñez Y et al (2010) Application of highly sensitive saturation labeling to the analysis of differential protein expression in infected ticks from limited samples. Proteome Sci 8:43
Sebatjane SI, Pretorius A, Liebenberg J et al (2010) In vitro and in vivo evaluation of five low molecular weight proteins of Ehrlichia ruminantium as potential vaccine components. Vet Immunol Immunopathol 137:217–225
Marcelino I, de Almeida AM, Ventosa M et al (2012) Tick-borne diseases in cattle: applications of proteomics to develop new generation vaccines. J Proteomics 75:4232–4250
Patramool S, Choumet V, Surasombatpattana P et al (2012) Update on the proteomics of major arthropod vectors of human and animal pathogens. Proteomics 12:3510–3523
Rachinsky A, Guerrero FD, Scoles G et al (2007) Differential protein expression in ovaries of uninfected and Babesia-infected southern cattle ticks, Rhipicephalus (Boophilus) microplus. Insect Biochem Mol Biol 37:1291–1308
Hill CA, Wikel SK (2005) The Ixodes scapularis genome project: an opportunity for advancing tick research. Trends Parasitol 21:151–153
Meyer JM, Kurtti TJ, Van Zee JP, Hill CA (2010) Genome organization of major tandem repeats in the hard tick, Ixodes scapularis. Chromosome Res 18:357–370
Villar M, Ayllón N, Busby AT et al (2010) Expression of heat shock and other stress response proteins in ticks and cultured tick cells in response to Anaplasma spp infection and heat shock. Int J Proteomics 2010:657261
Untalan PM, Guerrero FD, Haines LR, Pearson TW (2005) Proteome analysis of abundantly expressed proteins from unfed larvae of the cattle tick, Boophilus microplus. Insect Biochem Mol Biol 35:141–151
Vennestrøm J, Jensen PM (2007) Ixodes ricinus: the potential of two-dimensional gel electrophoresis as a tool for studying host-vector-pathogen interactions. Exp Parasitol 115:53–58
Wickramasekara S, Bunikis J, Wysocki V, Barbour AG (2008) Identification of residual blood proteins in ticks by mass spectrometry proteomics. Emerg Infect Dis 14:1273–1275
Tanaka K, Sawatani E, Shigueoka EM et al (2001) Isolation of bovine plasma albumin by liquid chromatography and its polymerization for use in immunohematology. Braz J Med Biol Res 34:977–983
Megger DA, Bracht T, Meyer HE, Sitek B (2013) Label-free quantification in clinical proteomics. Biochim Biophys Acta 1834:1581–1590
Bonzon-Kulichenko E, Pérez-Hernández D, Núñez E et al. (2011) A robust method for quantitative high-throughput analysis of proteomes by 18O labeling. Mol Cell Proteomics 10(1):M110.003335
Fic E, Kedracka-Krok S, Jankowska U et al (2010) Comparison of protein precipitation methods for various rat brain structures prior to proteomic analysis. Electrophoresis 31:3573–3579
Simpson DM, Beynon RJ (2010) Acetone precipitation of proteins and the modification of peptides. J Proteome Res 9:444–450
Acknowledgments
We thank the CMBSO proteomics facility (Centro de Biología Molecular Severo Ochoa, Madrid, Spain) for technical assistance. This research was supported by grants BFU2011-23896 and the EU FP7 ANTIGONE project number 278976. M. Popara is an early-stage researcher supported by the POSTICK ITN (postgraduate training network for capacity building to control ticks and tick-borne diseases) within the FP7-PEOPLE—ITN program (EU Grant No. 238511).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Popara, M., Villar, M., de la Fuente, J. (2015). Proteomics Characterization of Tick-Host-Pathogen Interactions. In: Cunha, M., Inácio, J. (eds) Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies. Methods in Molecular Biology, vol 1247. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2004-4_34
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2004-4_34
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2003-7
Online ISBN: 978-1-4939-2004-4
eBook Packages: Springer Protocols