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Biological Differences Between Native and Cultured Mesenchymal Stem Cells: Implications for Therapies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1235))

Abstract

We describe the current knowledge of the surface marker phenotype of native bone marrow mesenchymal stem/stromal cells (MSCs) in humans and in mouse models, highlighting similarities in the MSC marker “signature” between the two species. The chapter proceeds to discuss the published literature pertaining to native MSC topography and their interactions with hematopoietic stem cells and their progeny, as well as with blood vessels and nerve endings. Additionally, the chapter describes phenotypic and functional “drifts” that occur in MSC preparations as they are taken out of their native bone marrow microenvironment and induced to proliferate in vitro (in the presence of animal or human serum). We propose that the understanding of the biology of MSCs in their native niches in the bone marrow could lead to future developments in the treatment of hematological diseases such as multiple myeloma. Additionally, this knowledge would assist in the development of more “natural” MSC culture conditions, best preserving MSC functionality including their homing potential in order to optimize MSC transplantation in the context of graft-versus-host and other diseases.

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Authors and Affiliations

  1. Leeds Institute of Rheumatic and Musculoskeletal Medicine, Leeds University, Room 5.24 Clinical Sciences Building, Leeds, LS9 7TF, UK

    Elena Jones

  2. Institute of Transfusion Medicine and Immunohaematology, German Red Cross Blood Donor Service Baden-Württemberg-Hessen gGmbH, Johann-Wolfgang-Goethe University Hospital, Sandhofstrasse 1, D-60528, Frankfurt am Main, Germany

    Richard Schäfer

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Correspondence to Elena Jones .

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  1. HemoGenix, Inc., Colorado Springs, Colorado, USA

    Ivan N. Rich

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Jones, E., Schäfer, R. (2015). Biological Differences Between Native and Cultured Mesenchymal Stem Cells: Implications for Therapies. In: Rich, I. (eds) Stem Cell Protocols. Methods in Molecular Biology, vol 1235. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1785-3_10

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  • DOI: https://doi.org/10.1007/978-1-4939-1785-3_10

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