Abstract
Intercellular protein movement is an important mechanism in plant development. Here we present an integrated protocol that utilizes an inducible system to block plasmodesmata-dependent movement and assessment of fluorescent recovery after photobleaching (FRAP) to identify compounds that influence intercellular protein movement.
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Acknowledgments
The icals3m construct was part of the collaboration with the Y. Helariutta lab. S. Wu is supported by a grant from NSF (award # 1243945) to K. Gallagher.
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Wu, S., Gallagher, K.L. (2015). Techniques for Assessing the Effects of Pharmacological Inhibitors on Intercellular Protein Movement. In: Heinlein, M. (eds) Plasmodesmata. Methods in Molecular Biology, vol 1217. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1523-1_16
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DOI: https://doi.org/10.1007/978-1-4939-1523-1_16
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1522-4
Online ISBN: 978-1-4939-1523-1
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