Abstract
Adult bone marrow niche contains rare primitive but highly functional multipotent progenitors (e.g., mesenchymal stem cells; MSCs) capable of differentiating into specific mesenchymal tissues like bone, cartilage, muscle, fat tissues, ligament, dermis, bone marrow stroma, tendon, and other connective tissues. Upon in vivo transplantation, MSCs also secrete a wide range of growth factors, immunomodulatory cytokines, and important bioactive macromolecules including cell-derived exosomes to structure regenerative microenvironments. This protocol describes a mouse model to study bone formation/regeneration from adult mesenchymal stem cells.
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Acknowledgments
We are grateful to Department of Biotechnology (DBT), Government of India for Ramalingaswami Fellowship and research support grant (DBT Grant #BT/PR 15420/MED/31/122/2011) to SK.
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Kumar, S. (2014). Bone Defect Repair in Mice by Mesenchymal Stem Cells. In: Christ, B., Oerlecke, J., Stock, P. (eds) Animal Models for Stem Cell Therapy. Methods in Molecular Biology, vol 1213. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1453-1_16
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DOI: https://doi.org/10.1007/978-1-4939-1453-1_16
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