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Protocol for Cutaneous Wound Healing Assay in a Murine Model

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Stem Cells and Tissue Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1210))

Abstract

Cutaneous wound healing assay is important to address many key questions including (1) migration ability of different cells; (2) communication between the different cell types such as keratinocytes, fibroblasts, and immune cells; (3) understanding the cell-autonomous and non-cell-autonomous function(s) of the different cells; and (4) gene regulation in healing processes. Wound healing studies can be used to test new treatment modalities, function of new drugs/compounds, and stem cell-based therapies on the different stages of healing and for accelerating wound healing in patients with compromised healing. In this chapter, we have described a simple step-by-step protocol to generate full-thickness cutaneous wounds in the dorsal skin of mice, followed by collecting the post-wounding biopsied materials on specific days for histological and immunohistochemical analyses and for RNA and protein extractions.

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Acknowledgements

I would like to thank Xiaobo Liang and Shreya Bhattacharya for the images. I would like to also thank Arup K. Indra for critical reading of the review article. This work was supported by NIH grant 5R01AR056008-02.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, 1601 SW Jefferson str, Corvallis, OR, 97331, USA

    Gitali Ganguli-Indra

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  1. Gitali Ganguli-Indra
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Correspondence to Gitali Ganguli-Indra .

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Editors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, Oregon, USA

    Chrissa Kioussi

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Ganguli-Indra, G. (2014). Protocol for Cutaneous Wound Healing Assay in a Murine Model. In: Kioussi, C. (eds) Stem Cells and Tissue Repair. Methods in Molecular Biology, vol 1210. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1435-7_12

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  • DOI: https://doi.org/10.1007/978-1-4939-1435-7_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1434-0

  • Online ISBN: 978-1-4939-1435-7

  • eBook Packages: Springer Protocols

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