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Evaluation of Galectin Binding by Surface Plasmon Resonance

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Galectins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1207))

Abstract

Surface Plasmon Resonance (SPR) instruments, like the BIAcore 3000, are useful for studying the binding between macromolecules in real time. The high sensitivity and low sample consumption in the BIAcore enables the measurement of rapid kinetics and low affinities characteristic of many biological interactions. This chapter describes the affinity measurement of Galectins-1, -2, and -3 and their glycoside ligands using this approach.

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Acknowledgments

The author thanks Drs. Sean R. Stowell, Richard D. Cummings, and Richard Alvarez for helpful discussions during the course of this study. The BIAcore 3000 instrument used in this study was purchased with an NIH shared instrumentation grant awarded to Rodger P. McEver. The study was carried out in the Biacore Core Facility at the Oklahoma Medical Research Foundation.

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Authors and Affiliations

  1. Oklahoma Medical Research Center, 825 N.E. 13th Street, Oklahoma City, OK, 73104, USA

    Padmaja Mehta-D’souza

Authors
  1. Padmaja Mehta-D’souza
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Correspondence to Padmaja Mehta-D’souza .

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Editors and Affiliations

  1. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA

    Sean R. Stowell

  2. Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, Georgia, USA

    Richard D. Cummings

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Mehta-D’souza, P. (2015). Evaluation of Galectin Binding by Surface Plasmon Resonance. In: Stowell, S., Cummings, R. (eds) Galectins. Methods in Molecular Biology, vol 1207. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1396-1_7

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  • DOI: https://doi.org/10.1007/978-1-4939-1396-1_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1395-4

  • Online ISBN: 978-1-4939-1396-1

  • eBook Packages: Springer Protocols

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