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Customization of 13C-MFA Strategy According to Cell Culture System

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Metabolic Flux Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1191))

Abstract

13C-MFA is far from being a simple assay for quantifying metabolic activity. It requires considerable up-front experimental planning and familiarity with the cell culture system in question, as well as optimized analytics and adequate computation frameworks. The success of a 13C-MFA experiment is ultimately rated by the ability to accurately quantify the flux of one or more reactions of interest. In this chapter, we describe the different 13C-MFA strategies that have been developed for the various fermentation or cell culture systems, as well as the limitations of the respective strategies. The strategies are affected by many factors and the 13C-MFA modeling and experimental strategy must be tailored to conditions. The prevailing philosophy in the computation process is that any metabolic processes that produce significant systematic bias in the labeling pattern of the metabolites being measured must be described in the model. It is equally important to plan a labeling strategy by analytical screening or by heuristics.

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Author information

Authors and Affiliations

  1. Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Building 75, Corner of College and Cooper Road, Brisbane, QLD, 4072, Australia

    Lake-Ee Quek & Lars K. Nielsen

Authors
  1. Lake-Ee Quek
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  2. Lars K. Nielsen
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Corresponding author

Correspondence to Lars K. Nielsen .

Editor information

Editors and Affiliations

  1. Centre for Microbial Electrosynthesis (CEMES), Advanced Water Management Centre, University of Queensland, St. Lucia, Brisbane, Queensland, Australia

    Jens O. Krömer

  2. AIBN, University of Queensland, St. Lucia, Brisbane, Queensland, Australia

    Lars K. Nielsen

  3. Biology Department, RWTH Aachen University, Aachen, Germany

    Lars M. Blank

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Quek, LE., Nielsen, L.K. (2014). Customization of 13C-MFA Strategy According to Cell Culture System. In: Krömer, J., Nielsen, L., Blank, L. (eds) Metabolic Flux Analysis. Methods in Molecular Biology, vol 1191. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1170-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-1170-7_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1169-1

  • Online ISBN: 978-1-4939-1170-7

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