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Regulatory B Cells pp 127–141Cite as

Toll-Like Receptor Ligation for the Induction of Regulatory B Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1190))

Abstract

Toll-like receptors (TLRs) are key components for the recognition of microorganisms, for the initiation of innate immunity, and for promoting adaptive immune responses. TLR signaling in B cells, in addition to B cell receptor or CD40 ligation, plays an important role in B cell differentiation and activation. In contrast, various infectious agents and/or TLR ligands can also prime B cells to induce tolerance and downregulate inflammatory reactions; those B cells are called regulatory B (Breg) cells and are characterized by a dominant IL-10 production. Several studies have suggested that Breg cells are impaired in patients with autoimmune diseases and allergic asthma. However, the role for TLR ligands in the induction of Breg cells as a potential therapy for some of these inflammatory diseases has not yet been investigated. Here, we provide detailed instructions on how to analyze and validate cytokine production in human and mouse B cells in response to various TLR ligands. Furthermore, we describe an assay to investigate the suppressive properties of TLR-induced B cells to confirm their regulatory B cell status.

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Authors and Affiliations

  1. Department of Parasitology, Cellular Immunology of Helminths, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands

    Luciën E. P. M. van der Vlugt, Simone Haeberlein, Wouter de Graaf, Tyrill E. D. Martha & Hermelijn H. Smits Ph.D.

Authors
  1. Luciën E. P. M. van der Vlugt
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  2. Simone Haeberlein
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  3. Wouter de Graaf
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  4. Tyrill E. D. Martha
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  5. Hermelijn H. Smits Ph.D.
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Corresponding author

Correspondence to Hermelijn H. Smits Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Medical and Biological Sciences, University of Udine, Udine, Italy

    Gaetano Vitale

  2. Department of Medical and Biological Sciences, University of Udine, Udine, Italy

    Francesca Mion

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van der Vlugt, L.E.P.M., Haeberlein, S., de Graaf, W., Martha, T.E.D., Smits, H.H. (2014). Toll-Like Receptor Ligation for the Induction of Regulatory B Cells. In: Vitale, G., Mion, F. (eds) Regulatory B Cells. Methods in Molecular Biology, vol 1190. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1161-5_10

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  • DOI: https://doi.org/10.1007/978-1-4939-1161-5_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1160-8

  • Online ISBN: 978-1-4939-1161-5

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