Abstract
Recent studies have shown the ability of mesenchymal stem cells (MSCs) to migrate toward and engraft into the tumor sites, which provides a potential for their use as carriers for cancer gene therapy. Here, we describe the strategies of using MSCs as carriers for cancer gene therapy using a nonviral transfection method.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Dennis JE, Cohen N, Goldberg VM et al (2004) Targeted delivery of progenitor cells for cartilage repair. J Orthop Res 22:735–741
Studeny M, Marini FC, Dembinski JL et al (2004) Mesenchymal stem cells: potential precursors for tumor stroma and targeted-delivery vehicles for anticancer agents. J Natl Cancer Inst 96:1593–1603
Nakamura K, Ito Y, Kawano Y et al (2004) Antitumor effect of genetically engineered mesenchymal stem cells in a rat glioma model. Gene Ther 11:1155–1164
Nakamizo A, Marini F, Amano T et al (2005) Human bone marrow derived mesenchymal stem cells in the treatment of gliomas. Cancer Res 65:3307–3318
Kucerova L, Altanerova V, Matuskova M et al (2007) Adipose tissue-derived human mesenchymal stem cells mediated prodrug cancer gene therapy. Cancer Res 67:6304–6313
Yang X, Walboomers XF, van den Dolder J et al (2008) Non-viral bone morphogenetic protein 2 transfection of rat dental pulp stem cells using calcium phosphate nanoparticles as carriers. Tissue Eng Part A 14:71–81
Bisht S, Bhakta G, Mitra S et al (2005) pDNA loaded calcium phosphate nanoparticles: highly efficient non-viral vector for gene delivery. Int J Pharm 288:157–168
Ding W, Izumisawa T, Hattori Y et al (2009) Non-ionic surfactant modified cationic liposomes mediated gene transfection in vitro and in the mouse lung. Biol Pharm Bull 32:311–315
Huang YZ, Gao JQ, Chen JL et al (2006) Cationic liposomes modified with non-ionic surfactants as effective non-viral carrier for gene transfer. Colloids Surf B Biointerfaces 49:158–164
Pack DW, Hoffman AS, Pun S et al (2005) Design and development of polymers for gene delivery. Design and development of polymers for gene delivery. Nat Rev Drug Discov 4:581–593
Jo J, Ikai T, Okazaki A et al (2007) Expression profile of plasmid DNA by spermine derivatives of pullulan with different extents of spermine introduced. J Control Release 118:389–398
Hu YL, Huang B, Zhang TY et al (2012) Mesenchymal stem cells as a novel carrier for targeted delivery of gene in cancer therapy based on nonviral transfection. Mol Pharm 9:2698–2709
Zhang TY, Huang B, Yuan ZY et al (2013) Gene recombinant bone marrow mesenchymal stem cells as a tumor-targeted suicide gene delivery vehicle in pulmonary metastasis therapy using non-viral transfection. Nanomedicine 2013 10:257–67
Acknowledgement
This work was financially supported by National Natural Science Foundation of China (30873173 and 81273441 to Jian-Qing Gao, 81001410 to Yu-Lan Hu), Zhejiang Provincial Natural Science Foundation of China (Y13H300002 to Yu-Lan Hu), and Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, New York
About this protocol
Cite this protocol
Hu, YL., Gao, JQ. (2014). Recombinant Stem Cells as Carriers for Cancer Gene Therapy. In: Jain, K. (eds) Drug Delivery System. Methods in Molecular Biology, vol 1141. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0363-4_5
Download citation
DOI: https://doi.org/10.1007/978-1-4939-0363-4_5
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0362-7
Online ISBN: 978-1-4939-0363-4
eBook Packages: Springer Protocols