Abstract
Rapid fractionation by membrane filtration was used to determine ATP/ADP ratios in chloroplasts, mitochondria and cytosol of barley protoplasts incubated under different conditions.
In darkness the ATP/ADP ratio was higher in the cytosol than in chloroplasts and mitochondria. In the light at saturating CO2 the ATP/ADP ratio increased in chloroplasts and mitochondria compared to darkness whereas a slight decrease was observed in the cytosol. In the light at limiting CO2 the ATP/ADP ratio increased in all compartments compared to saturating CO2. The cytosolic ATP/ADP ratio is higher in the light at limiting CO2 compared to darkness. As CO2 is limiting for photosynthesis in C3 plants at atmospheric conditions this has important implications for the regulation of the metabolism in the cytosol in the light.
When photosynthesis was reduced by lack of CO2 the cytosolic ATP/ADP ratio was much higher than when photosynthesis was limited by inhibition of the Calvin cycle. From this it is suggested that, under certain conditions, photorespiration can function to shuttle ATP from the chloroplasts into the cytosol.
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References
Bergman, A., and Ericson, I., 1983, Effects of pH, NADH, succinate, and malate on the oxidation of glycine in spinach leaf mitochondria, Physiol. Plant. 59:421.
Bruinsma, J., 1986, A comment on the spectrophotometric determination of chlorophyll, Biochim. Biophys. Acta. 52:576.
Day, D. A., and Wiskich, J. T., 1984, Transport processes of isolated mitochondria, Physiol. Veg. 22:241.
Dry, S. B., Day, D. A., and Wiskich, J. T., 1983, Preferential oxidation of glycine by the respiratory chain of pea leaf mitochondria, FEBS Lett. 158:154.
Flügge, V. I. and Heldt, H. W., 1984, The phosphate — triose phosphate — phosphoglycerate translocator of the chloroplasts, Trends Biochem. Sci. 9:530.
Gardeström, P., and Edwards, G. E., 1983, Isolation of mitochondria from leaf tissue of Panicum milineceum, a NAD-malic enzyme type C4 plant, Plant Physiol. 71:24.
Gardeström, P., and Edwards, G. E., 1985, Leaf mitochondria (C3+C4+CAM), in: Encyclopedia of Plant Physiology, New Series, Vol. 18:314. R. Douce and D. A. Day, eds, Springer Verlag, Berlin.
Hampp, R., Goller, M., and Ziegler, H., 1982, Adenylate levels, energy charge, and phosphorylation potential during dark-light and light-dark transition in chloroplasts, mitochondria and cytosol of mesophyll protoplasts from Avena sativa L., Plant Physiol. 69:448.
Hatch, M. D., Dröscher, L., Flügge, U. I., and Heldt, H. W., 1984, A specific translocator for oxaloacetate in chloroplasts, FEBS Lett. 178:15.
Hatch, M. D., 1978, A simple spectrophotometric assay for fumarase hydratase in crude tissue extract, Analyt. Biochem. 85:271.
Lilley, R. McC, Stitt, M., Mader, G., and Heldt, H. W., 1982, Rapid fractionation of wheat leaf protoplasts using membrane filtration, Plant Physiol. 70:965.
Nishimura, M., Douce, R., and Akazawa, T., 1985, A simple method for estimating intactness of spinach leaf protoplasts by glycolate oxidase assay, Plant Physiol. 78:343.
Pradet, A., and Raymond, P., 1983, Adenine nucleotide ratios and adenylate energy charge in energy metabolism, Ann. Rev. Plant Physiol. 34:199.
Santarius, K. A., and Heber, U., 1965, Changes in the intracellular levels of ATP, ADP, AMP and Pi and regulatory function of the adenylate system in leaf cells during photosynthesis, Biochim. Biophys. Acta. 102:39.
Sicher, R. C, 1984, Glycolaldehyde inhibition of photosynthetic carbon assimilation by isolated chloroplasts and protoplasts, in: Advances in Photosynthesis Research, Vol III, 6:413. C. Sybesma, ed., Martinus Nijhoff, The Hague.
Stitt, M., Lilley, R. McC, and Heldt, H. W., 1982, Adenine nucleotide levels in the cytosol, chloroplasts, and mitochondria of wheat leaf protoplasts, Plant Physiol. 70:971.
Winter, K., Foster, J. G., Edwards, G. E., and Holtum, J. A. M., 1982, Intracellular location of enzymes of carbon metabolism in Mesembryanthemum crystallinum exhibiting C3 photosynthetic characteristics or performing crassulacean acid metabolism, Plant Physiol. 69:300.
Woo, K.C, and Osmond, C. B., 1976, Glycine decarboxylation in mitochondria isolated from spinach leaves, Aust. J. Plant Physiol. 3:771.
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Gardeström, P. (1987). The Effect of Light on Mitochondrial Respiration Studied by Rapid Fractionation of Protoplasts. In: Moore, A.L., Beechey, R.B. (eds) Plant Mitochondria. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3517-5_28
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DOI: https://doi.org/10.1007/978-1-4899-3517-5_28
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