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Multidimensional Fluorescence Microscopy: Optical Distortions in Quantitative Imaging of Biological Specimens

  • Chapter
Fluorescence Microscopy and Fluorescent Probes

Abstract

Photometric and morphometric measurement of biological microscopy specimens often poses problems due to their optical properties and those of the microscope (Fricker and White, 1992). 3-D Visualisation (White, 1995) and quantification (Sandison et al, 1995, Taylor and Wang, 1989b) of fluorescent probes in living tissue is possible by computer assisted confocal microscopy (e. g. Brackenhoff et al, 1979; Shotton, 1989; Wilson, 1990, Inoué 1995). The specimen is both the object under investigation and also a key component of this integrated imaging system.

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Author information

Authors and Affiliations

  1. Plant Sciences Department, Oxford University, South Parks Rd, Oxford, OX1 3RB, UK

    N. S. White, M. D. Fricker & J. L. Wood

  2. Physiology Department, Oxford University, Parks Rd, Oxford, OX1 3PT, UK

    R. J. Errington

Authors
  1. N. S. White
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  2. R. J. Errington
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  3. M. D. Fricker
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  4. J. L. Wood
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Editor information

Editors and Affiliations

  1. Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic

    Jan Slavík

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© 1996 Springer Science+Business Media New York

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White, N.S., Errington, R.J., Fricker, M.D., Wood, J.L. (1996). Multidimensional Fluorescence Microscopy: Optical Distortions in Quantitative Imaging of Biological Specimens. In: Slavík, J. (eds) Fluorescence Microscopy and Fluorescent Probes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1866-6_4

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  • DOI: https://doi.org/10.1007/978-1-4899-1866-6_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1868-0

  • Online ISBN: 978-1-4899-1866-6

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