Abstract
The choroid plexuses, which comprise the blood-cerebrospinal fluid (CSF) barrier, are situated within the third, fourth, and lateral cerebral ventricles (Fig. 1). The choroid plexus functions in the secretion and reabsorption of a variety of compounds and is similar in morphology to other epithelia that are involved in such transport processes (i. e., kidney and intestine) (Spector and Johanson, 1989). The choroid plexus villi are composed of a single layer of simple columnar or cuboidal epithelial cells that rest on a basement membrane. Similar to other epithelia, the choroid plexus cell is polar, having distinct brush border (faces the CSF) and basolateral (faces the blood) surfaces. Each polar membrane has distinct characteristics, and, in particular, transporters are uniquely distributed between the two surfaces. This polar arrangement allows for the net secretion of solutes and water from the blood to the CSF as well as the net reabsorption of other compounds in the opposite direction, from CSF to blood. In addition, the epithelial cells of the choroid plexus are laterally joined to each other by a continuous band of tight junctions (zonulae occludens) located near the brush border surface. This prevents significant paracellular movement of solutes into and out of the CSF via the choroid plexus.
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References
Anderson, B. D., Hoesterey, B. L., Baker, D. C., and Galinsky, R. E., 1990, Uptake kinetics of 2′,3′-dideoxyinosine into brain and cerebrospinal fluid of rats: Intravenous infusion studies, J. Pharmacol Exp. Ther. 253:113–118.
Artursson, P., and Magnusson, C., 1990, Epithelial transport of drugs in cell culture. II: Effect of extracellular calcium concentration on the paracellular transport of drugs of different lipophilicities across monolayers of intestinal epithelial (Caco-2) cells, J. Pharm. Sci. 79:595–600.
Bradford, M. M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72:248–254.
Carter, S. C., and Kimmich, G. A., 1979, Membrane potentials and sugar transport by ATP-depleted intestinal cells: Effect of anion gradients, Am. J. Physiol. 237:C67–C74.
Chung, S.-J., Ramanathan, V., Giacomini, K., and Brett, C., 1994, Characterization of a sodium-dependent taurine transporter in rabbit choroid plexus, Biochim. Biophys. Acta 1193:10–16.
Crook, R. B., Kasagami, H., and Prusiner, S. B., 1981, Culture and characterization of epithelial cells from bovine choroid plexus, J. Neurochem. 37:845–854.
Freshney, R. I. (ed.), 1986, Animal Cell Culture: A Practical Approach, IRL Press, Oxford.
Hofer, M., and Hogget, J. G., 1980, Transport across Biological Membranes, Pitman Advanced Publishing Program, Boston.
Kim, C. S., and Pritchard, J. B., 1993, Transport of 2,4,5-trichlorophenoxyacetic acid across the blood-cerebrospinal fluid barrier of the rabbit, J. Pharmacol. Exp. Ther. 267:751–757.
Kragh, H. U., Roigaard, P. H., Jacobsen, C., and Sheikh, M. I., 1984, Renal transport of neutral amino acids. Tubular localization of Na+-dependent phenylalanine-and glucose-transport systems, Biochem. J. 220:15–24.
Le Hir, M., and Dubach, U. C., 1985a, Concentrative transport of purine nucleosides in brush border vesicles of the rat kidney, Eur. J. Clin. Invest. 15:121–127.
Le Hir, M., and Dubach, U. C., 1985b, Uphill transport of pyrimidine nucleosides in renal brush border vesicles, Pflügers Arch. 404:238–243.
Mayer, S. E., and Sanders, B. E., 1993, Sodium-dependent antiporters in choroid plexus epithelial cultures from rabbit, J. Neurochem. 60:1308–1316.
Merlis, J. K., 1940, The effect of changes in the calcium content of the cerebrospinal fluid on spinal reflex activity in the dog, Am. J. Physiol. 131:67–72.
Neuwelt, E. A. (ed.), 1989, Implications of the Blood Brain Barrier and Its Manipulation, Plenum Medical Book Co., New York.
Pardridge, W. M., Boado, R. J., Black, K. L., and Cancilla, P. A., 1992, Blood-brain barrier and new approaches to brain drug delivery [see comments], West. J. Med. 156:281–286.
Pollay, M., 1975, Formation of cerebrospinal fluid. Relation of studies of isolated choroid plexus to the standing gradient hypothesis, J. Neurosurg. 42:665–673.
Ross, H. J., and Wright, E. M., 1984, Neutral amino acid transport by plasma membrane vesicles of the rabbit choroid plexus, Brain Res. 295:155–160.
Semenza, G., 1982, The Na+/D-glucose co-transporter of the small-intestinal brush-border membrane, Biochem. Soc. Trans. 10:7 (Abstract).
Southwell, B. R., Duan, W., Alcorn, D., Brack, C., Richardson, S. J., Kohrle, J., and Schreiber, G., 1993, Thyroxine transport to the brain: Role of protein synthesis by the choroid plexus, Endocrinology 133:2116–2126.
Spector, R., 1982, Nucleoside transport in choroid plexus: Mechanism and specificity, Arch. Biochem. Biophys. 216:693–703.
Spector, R., 1987, Hypoxanthine transport through the blood-brain barrier, Neurochem. Res. 12:791–796.
Spector, R., and Boose, B., 1979, Active transport of riboflavin by the isolated choroid plexus in vitro, J. Biol. Chem. 254:10286–10289.
Spector, R., and Eells, J., 1984, Deoxynucleoside and vitamin transport into the central nervous system, Fed. Proc. 43:196–200.
Spector, R., and Johanson, C. E., 1989, The mammalian choroid plexus, Sci. Am. 261:68–74.
Stein, W. D. (ed.), 1990, Channels, Carriers, and Pumps, Academic Press, San Diego.
Taub, M., 1985, Tissue Culture of Epithelial Cells, Plenum Press, New York.
Wainer, I. W. (ed.), 1993, Drug Stereochemistry: Analytical Methods and Pharmacology, Marcel Dekker, New York.
Whittico, M. T., Gang, Y. A., and Giacomini, K. M., 1990, Cimetidine transport in isolated brush border membrane vesicles from bovine choroid plexus, J. Pharmacol. Exp. Ther. 255:615–623.
Whittico, M. T., Hui, A. C., and Giacomini, K. M., 1991, Preparation of brush border membrane vesicles from bovine choroid plexus, J. Pharmacol. Methods 25:215–227.
Wilson, G., 1990, Cell culture techniques for the study of drug transport, Eur. J. Drug Metab. Pharmacokinet. 15:159–163.
Wu, X., Yuan, G., Brett, C. M., Hui, A. C., and Giacomini, K. M., 1992, Sodium-dependent nucleoside transport in choroid plexus from rabbit. Evidence for a single transporter for purine and pyrimidine nucleosides, J. Biol. Chem. 267:8813–8818.
Wu, X., Gutierrez, M. M., and Giacomini, K. M., 1994, Further characterization of the sodium-dependent nucleoside transporter (N3) in choroid plexus from rabbit, Biochim. Biophys. Acta 1191:190–196.
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Washington, C.B., Giacomini, K.M., Brett, C.M. (1996). Methods to Study Drug Transport in Isolated Choroid Plexus Tissue and Cultured Cells. In: Borchardt, R.T., Smith, P.L., Wilson, G. (eds) Models for Assessing Drug Absorption and Metabolism. Pharmaceutical Biotechnology, vol 8. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1863-5_14
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