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Fluorescence Spectroscopic Studies of Proteins

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Proteins: Structure, Function, and Engineering

Part of the book series: Subcellular Biochemistry ((SCBI,volume 24))

Abstract

Fluorescence spectroscopy has emerged as one of the most important tools in the study of protein structure and function. Sensitivity of the fluorescence techniques is a major advantage. Although it cannot provide total structure like X-ray crystallography or nuclear magnetic resonance (NMR), the sensitivity of the technique allows one to work at or near the intracellular concentrations of macro-molecules and ligands. This is particularly important for proteins that self-associate at higher concentrations. Although steady-state fluorescence spectra can now be easily obtained and steady-state fluorometers are inexpensive and widely available, they suffer from several disadvantages.

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Author information

Authors and Affiliations

  1. Department of Biophysics, Bose Institute, Calcutta, 700 054, India

    Siddhartha Roy

  2. Department of Biochemistry, Bose Institute, Calcutta, 700 054, India

    B. Bhattacharyya

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  1. Siddhartha Roy
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  2. B. Bhattacharyya
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Editors and Affiliations

  1. Department of Biophysics, Bose Institute, Calcutta, 700054, India

    B. B. Biswas  & Siddhartha Roy  & 

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Roy, S., Bhattacharyya, B. (1995). Fluorescence Spectroscopic Studies of Proteins. In: Biswas, B.B., Roy, S. (eds) Proteins: Structure, Function, and Engineering. Subcellular Biochemistry, vol 24. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1727-0_4

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  • DOI: https://doi.org/10.1007/978-1-4899-1727-0_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1729-4

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