Abstract
In vitro susceptibility testing of Trichomonas vaginalis has been performed by many authors.1–10 Most have adapted the classical broth macrodilution methodology to test tubes or microwell plates. However, Lossick11 has criticized susceptibility testing of T. vaginalis for its failure to predict treatment outcome in patients and for lack of standardized practical procedures. For example, some investigators employed an incubation time period that did not correlate with the growth curve of the protozoan,4,10 had no negative growth control,1–10 did not use a standardized inoculum8,10 and/or required an additional subculturing for determination of Minimum Lethal Concentration (MLC). 2,3,5,6,9,10
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References
H.L. Howes, J. E. Lynch, and J. L. Kivlin. 1970. Tinidazole, a new antiprotozoal agent: effect on Trichomonas and other protozoa. Antimicrob. Agents Chemother. 1969: 261–266.
R. Nielsen. 1973. Trichomonas vaginalis II. Laboratory investigations in trichomoniasis. Brit. J. Vener. Dis. 49: 531–535.
B. Korner and H. K. Jensen. 1976. Sensitivity of Trichomonas uaginalis to metronidazole, tinidazole and nifuratel in vitro. Brit. J. Vener. Dis. 52: 404–408.
R.F. Smith and A. Di Domenico. 1980. Measuring the in vitro susceptibility of Trichomonas vaginalis to metronidazole. A disk broth method. Sex. Transm. Dis. 7: 120–124.
E.D. Ralph, R. Darwish, T. W. Austin, E. A. Smith, and F. L. M. Pattison. 1983. Susceptibility of Trichomonas vaginalis strains to metronidazole: response to treatment. Sex. Transm. Dis. 10: 119–122.
J.N. Krieger, C. S. Dickins, and M. F. Rein. 1985. Use of a time-kill technique for susceptibility testing of Trichomonas uaginalis. Antimicrob. Agents Chemother. 27: 332–336.
J.A. Escario, A. M. Igea, M. Contreras, A. R. Martinez-Fernandez, R. Claramunt, and C. Lopez. 1988. Antiparasitic activity of nine pyrazole derivatives against Trichomonas uaginalis, Entamoeba invaders, and Plasmodium berghei. Ann. Trop. Med. Parasitol. 32: 257–262.
W.H.R. Lumsden, C. Harrison, and D. H. H. Robertson. 1988. Treatment failure of Trichomonas uaginalis infections in females. II. In vitro estimation of the sensitivity of the organism to metronidazole. J. Antimicrob. Chemother. 21: 555564.
M.J. Muller, G. Lossick, and T. E. Gorrell. 1988. In vitro susceptibility of Trichomonas vaginalis to metronidazole and treatment outcome in vaginal trichomoniasis. Sex. Transco. Dis. 15: 17–24.
S.D. Sears and J. O’Hare. 1988. In vitro susceptibility of Trichomonas vaginalis to 50 antimicrobial agents. Antimicrob. Agents Chemother. 32: 144–146.
J.G. Lossick. 1990. Therapy of urogenital trichomoniasis, p. 324–341. In B. M. Honigberg (ed.), Trichomonads parasitic in humans. Springer-Verlag, New York.
B.J. Bromke and M. Furiga. 1991. Carrageenan is a desirable substitute for agar in media growing Trichomonas uaginalis. J. Microb. Methods 13: 61–65.
B.J. Bromke. 1986. A serum-free, lipid-supplemented medium for the growth of Trichomonas uaginalis. J. Microb. Methods 6: 55–59.
G.E. Garber and L. T. Lemchuk-Favel. 1989. Characterization and purification of extracellular proteases of Trichomonas vaginalis. Can. J. Microbiol. 35: 903–909.
G. Wagner and R. Levin. 1978. Oxygen tension of the vaginal surface during sexual stimulation in the human. Fert. Steril. 30: 50–53.
D.L. Linstead. 1990. Cultivation, p. 91–111. In B. M. Honigberg (ed.), Trichomonads parasitic in humans. Springer-Verlag, New York.
D.H. Robertson, R. Heyworth, C. Harrison and W. H. R. Lumsden. 1988. Treatment failure in Trichomonas uaginalis infections in females. I. Concentrations of metronidazole in plasma and vaginal content during normal and high dosage. J. Antimicrob. Chemother. 21: 373–378.
J.A. McFadzean, I. M. Pugh, S. L. Squires, and J. P. F. Whelan. 1969. Further observations on strain sensitivity of Trichomonas vaginalis to metronidazole. Brit. J. Vener. Dis. 45: 161–162.
P. Sithithaworn, J. Sithithaworn, K. Kitjaroen, C. Pairojkul, S. Ratananurakpong, and T. Sriswangwonk. 1989. Comparative in vitro sensitivity to metronidazole of Trichomonas vaginalis with isolates and clones. Southeast Asian J. Trop. Med. Publ. H. 20: 279–284.
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Bromke, B.J., Furiga, M.C., Hendershot, R.C., McGinn, M. (1995). An Improved Method for in Vitro Susceptibility Testing of Trichomonas Vaginalis . In: Jungkind, D.L., Mortensen, J.E., Fraimow, H.S., Calandra, G.B. (eds) Antimicrobial Resistance. Advances in Experimental Medicine and Biology, vol 390. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9203-4_18
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