Summary
Thirty-five sera with binding greater than 20% in a myeloperoxidase (MPO, Calbiochem) ELISA were tested in Western blot analyses. 5/35 blotted MPO, but 5/35 blotted lactoferrin (LF) contaminating the commercial MPO preparation. All the antiLF positive sera, but none of the anti-MPO positive sera, also exhibited anti-nuclear binding on Hep2 cells. Three of the patients with anti-LF antibodies had vasculitis affecting areas additional to the pulmonary-renal involvement which characterised the patients with anti-MPO antibodies.
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References
Andrews P.C. and Krinsky N.I., 1986, Human myeloperoxidase and hemi-peroxidase. Methods in Enzymology, 132: 369–378.
Jayne D.R.W., Marshall P.D., Jones S.J., and Lockwood C.M., 1990, Autoantibodies to GBM and neutrophil cytoplasm in rapidly progressive glomerulonephritis, Kidney Int. 37: 965–970.
Esnault V.L.M., Jayne D.R.W., Weetman A.P., and Lockwood C.M., 1991, IgG subclass distribution and relative functional affinity of anti-myeloperoxidase antibodies in systemic vasculitis at presentation and during follow-up, Immunology, 74: 714–718.
Esnault V.L.M., Mathieson P.W., Thiru S., Oliveira D.B.G., and Lockwood C.M., 1992, Autoantibodies to myeloperoxidase in Brown Norway rats treated with mercuric chloride, Laboratory Investigation 67: 114–120.
Savage C.O.S., Winearls C.G., Jones S., Marshall P.D., and Lockwood C.M., 1987, Prospective study of radioimmunoassay for antibodies against neutrophil cytoplasm in diagnosis of systemic vasculitis, Lancet 1: 1389–1393.
Schägger H. and Von Jagow G., 1987, Tricine-Sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Anal. Biochem. 166: 368–379.
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© 1993 Springer Science+Business Media New York
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Esnault, V.L.M., Short, A.K., Jones, S.J., Skehel, M., Walker, J., Lockwood, C.M. (1993). Lactoferrin Co-Purifies with Myeloperoxidase and is Recognised by Anti-Neutrophil Cytoplasm Antibodies. In: Gross, W.L. (eds) ANCA-Associated Vasculitides. Advances in Experimental Medicine and Biology, vol 336. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9182-2_16
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DOI: https://doi.org/10.1007/978-1-4757-9182-2_16
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