Skip to main content
  • 361 Accesses

Abstract

A new molecular ionization technique known as matrix-assisted laser desorption/ionization (MALDI) was first reported by two groups in 1988 1.2. This ionization technique is most often coupled with the simplest form of mass spectrometry (MS), time-of-flight (TOF). The advantages of this ionization technique were quickly recognized and commercially available MALDI-MS instruments started to appear in 1991. Now, after significant improvements in instrumentation MALDI-MS has become a common analytical technique. While MALDI-MS is still relatively unknown by food scientists, it offers significant advantages over other analytical methods for food analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. K. Tanaka, H. Waki, Y. Ido, S. Akita, Y. Yoshida and T. Yoshida. 1988. Protein and polymer analysis up to m/z 100,000 by laser ionization time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 2: 151.

    Article  CAS  Google Scholar 

  2. M. Karas and F. Hillenkamp. 1988. Laser desorption ionization of proteins with molecular masses exceeding 10,000 Daltons. Anal. Chem. 60: 2299.

    Article  CAS  Google Scholar 

  3. J.A. Carrol and R.C. Beavis. 1997. Matrix-assisted Laser Desorption and Ionization, Chapter 7 in: “Experimental Methods in Physical Sciences” J.C. Miller and R.F. Haglund, Jr., eds. - available on the internet: http//128.122.10.3/MALDI/ChemPhysMan.htm

    Google Scholar 

  4. D.C. Shriemer and L. Li. 1996. Detection of high molecular weight narrow polydispersed polymers up to 1.5 million Daltons by MALDI mass spectrometry. Anal. Chem. 68: 2721.

    Article  Google Scholar 

  5. T. Souki, J.A. Marto, F.M. White, S. Guan and A.G. Marshall. 1995. Attomole biomolecule mass analysis by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance. Anal. Chem. 67: 4139.

    Article  Google Scholar 

  6. M.W. Duncan, G. Matanovic and A. Cerpa-Poljak. 1993. Quantitative analysis of low molecular weight compounds of biological interest by matrix-assisted laser desorption ionization. Rapid Commun. Mass Spectrom. 7: 1090.

    Article  CAS  Google Scholar 

  7. W.R. Wilkinson, A.I. Gusev, A. Proctor, M Houalla, D.M. Hercules. 1997. Selection of internal standards for quantitative analysis by matrix-assisted laser desorption-ionization (MALDI) time-of-flight mass spectrometry. Fresenius J. Anal. Chem. 357: 241.

    Article  CAS  Google Scholar 

  8. R. Kaufmann, B. Spengler and F. Lützenkirchen. 1993. Mass spectrometric sequencing of linear peptides by product-ion analysis in a reflectron time-of-flight mass spectrometer using matrix-assisted laser desorption ionization. Rapid Commun. Mass Spectrom. 7: 902.

    Article  CAS  Google Scholar 

  9. M.J. Vestal, P. Juasz and S.A. Martin. 1995 Delayed extraction matrix-assisted laser desorption time-offlight mass spectrometry. Rapid Commun. Mass Spectrom. 9: 1044.

    Article  CAS  Google Scholar 

  10. R.S. Brown and J.J. Lennon. 1995. Mass resolution improvement by incorporation of pulsed ion extraction in a matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometer. Anal. Chem. 67: 1998.

    Google Scholar 

  11. O.N. Jensen, A. Podtelejnkov and M. Mann. 1996. Delayed extraction improves specificity in database searches by matrix-assisted laser desorption/ionization peptide maps. Rapid Commun. Mass Spectrom. 10: 1371.

    Article  CAS  Google Scholar 

  12. D.R. Hickman, P. Roepstorff, P.R. Shewry and A.S. Tatham. 1995. Molecular weights of high molecular weight subunits of glutenin determined by mass spectrometry. J. Cereal Sci. 22: 99.

    Article  CAS  Google Scholar 

  13. E. Méndez, E. Camafeita, J.S. Sebastieân, 1. Valle, J. Solis, F.J. Mayer-Posner, D. Suckau, C. Marfisi and F. Soriano. 1995. Direct identification of wheat gliadins and related cereal prolamins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J. Mass Spect. 199 Suppl.: S123.

    Google Scholar 

  14. S. Catinella, P. Traldi, C. Pinelli, E. Dallaturca and R. Marsilio. 1996. Matrix-assisted laser desorption/ionization mass spectrometry in milk science. Rapid Commun. Mass Spectrom. 10: 1629.

    Article  CAS  Google Scholar 

  15. T. Krishnamurthy and P.L. Ross. 1996. Rapid identification of bacteria by direct matrix-assisted laser desorption/ionization mass spectrometric analysis of whole cells. Rapid Commun. Mass Spectrom. 10: 1992.

    Article  CAS  Google Scholar 

  16. M.A. Claydon, S.N. Davey, V. Edwards-Jones and D.B. Gordon. 1996. The rapid identification of intact microorganisms using mass spectrometry. Nature Biotech’’, November: 1584.

    Google Scholar 

  17. A.M. Gouldsworthy, J. Leaver and J.M. Banks. 1996. Application of a mass spectrometry sequencing technique for identifying peptides present in cheddar cheese. Int. Dairy J. 6: 781.

    Article  CAS  Google Scholar 

  18. T.M. Billeci and J.T. Stults. 1993. Tryptic mapping of recombinant proteins by matrix-assisted laser desorption/ionization mass spectrometry. Anal. Chem. 65: 1709.

    Article  CAS  Google Scholar 

  19. J.S. Cottrel, M. Koerner and R. Gerhards. 1995. Characterization of synthetic polymers by matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrum. 9: 1562.

    Article  Google Scholar 

  20. M.D. Mohr, K.O. Bömsen and M Widmer. 1995. Matrix-assisted laser desorption/ionization mass spectrometry: improved matrix for oligosaccharides. Rapid Commun. Mass Spectrom. 9: 809.

    Article  CAS  Google Scholar 

  21. P. Chen, A.G. Baker and M.V. Novotey. 1997. The use of osazones as matrices for the matrix-assisted laser desorption/ionization mass spectrometry of carbohydrates. Anal. Biochern. 244: 144.

    Article  CAS  Google Scholar 

  22. J.J. Pitt and J.J. Gorman. 1997. Oligosaccharide characterization and quantitation using 1-phenyl-3-methyl5-pyrazolone derivatization and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal. Biochem. 248: 63.

    Article  CAS  Google Scholar 

  23. M.C. Fitzgerald and L.M. Smith. 1995. Mass spectrometry of nucleic acids: the promise of matrix-assisted laser desorption-ionization (MALDI) mass spectrometry. Annu. Rev. Biophvs. Biomol. Struct. 24: 117.

    Article  CAS  Google Scholar 

  24. S.G. Penn, M.T. Cancilla, M.K. Green and C.B. Lerilla. 1997. Direct comparison of matrix assisted laser desorption/ionization and electrospray ionization in the analysis of gangliosides by Fourier transform mass spectrometry.European Mass Spectr. 3: 67.

    Article  CAS  Google Scholar 

  25. S. Gerdt, G. Lochnit, R.D. Dennis and R. Geyer. 1997. Isolation and structural analysis of three neutral glycosphingolipids from a mixed population of caenorhabditi.s elegans. Glvcobiol. 7: 265.

    CAS  Google Scholar 

  26. E. Pittenauer, E.R. Schmid, G. Allmainer, L. Puchinger and E. Kienzl. 1996. Sample preparation for the analysis of glycerophospholipids by matrix-assisted positive and negative ion Cf 252 plasma desorption time of flight mass spectrometry. European Mass Spectr. 2: 247.

    Article  CAS  Google Scholar 

  27. D.J. Harvey. 1995. Matrix-assisted laser desorption/ionization mass spectrometry ofphospholipids. J. Mass Speck: 30: 1333.

    Article  CAS  Google Scholar 

  28. M. Onishi-Kameyama, A. Yanagida, T. Kanada and T. Nagata. 1997. Identification of catechin oligomers from apple (Malus pumila cv. Fuji) in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and fast-atom bombardment mass spectrometry. Rapid Commun. Mass Speck: 11: 31.

    Article  Google Scholar 

  29. R. Lidgard and M.W. Duncan. 1995. Utility of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the analysis of low molecular weight compounds. Rapid Commun. Mass Specb: 9: 128.

    Article  CAS  Google Scholar 

  30. I.L. Koumenis, M.L. Vestal, A.L. Yergey, S. Abrams, S.N. Deming and T.W. Hutchens. 1995. Quantitation of metal isotope ratios by laser desorption time-of-flight mass spectrometry. Anal. Cheer. 40: 2533.

    Google Scholar 

  31. D.C. Abell and P. Sporns. 1996. Rapid quantitation of potato glycoalkaloids by matrix-assisted laser desoption/ionization time-of-flight mass spectrometry. J. Agric. Food Chem. 44: 2292.

    Article  CAS  Google Scholar 

  32. T. Wingerath, W. Stahl, D. Kirsch, R. Kaufmann and H. Sies. 1996. Fruit juice carotenol fatty acid ester and carotenoids as identified by matrix-assisted laser desorption ionization (MALDI) mass spectrometry. J. Agric. Food Cheer. 44: 2006.

    Google Scholar 

  33. B.L. Ackermann, B.T. Regg, L. Colombo, S. Stella and J.E. Coutant. 1996. Rapid analysis of antibiotic-containing mixtures from frementation broths by using liquid chromatography - electrospray ionization - mass spectrometry and matrix-assisted laser desorption ionization - time-of-flight - mass spectrometry. J. Amer Soc Mass Spect. 7: 1227.

    Article  CAS  Google Scholar 

  34. D.C. Muddiman, A.I. Gusev, K. Stoppek-Langer, A. Proctor, D.M. Hercules, P. Tat, R. Venkatararmanan and W. Diven. 1995. Simultaneous quantification of cyclosporin A and its major metabolites by time-offlight secondary-ion mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry utilizing data analysis techniques: comparison with high-performance liquid chromatrography. J. Mass Spec. 30: 1469.

    Article  CAS  Google Scholar 

  35. T. Wingerath, W. Stahl, D. Kirsch, R. Kaufmann and H. Sies. 1996. Fruit juice carotenol fatty acid ester and carotenoids as identified by matrix-assisted laser desorption ionization (MALDI) mass spectrometry. J..4gric. Food Chem. 44: 2006.

    Google Scholar 

  36. D. Rideout, A. Bustamante and G. Siuzdak. 1993. Cationic drug analysis using matrix-assisted laser desorption/ionization mass spectrometry: application to influx kinetics, multidrug resistance and intracellular chemical change. Proc. Natl. Acad. Sci. USA. 90: 10226.

    Article  CAS  Google Scholar 

  37. A.H. Brockman and R. Orlando. 1995. Probe-immobilized affinity chromatography/mass spectrometry. Anal. Cheer. 67: 4581.

    Article  CAS  Google Scholar 

  38. R.P. Pauly, F. Rosche, M. Wermann, C.H.S. Mcintosh, R.A. Pederson and H.U. Demuth. 1996. Investigation of glucose-dependent insulinotropic polypeptide-(1–42) and glucagon-like peptide-1-(7–36) degradation in Vitro by dipeptidyl peptidase IV using matrix-assisted laser desorption/ionization-time of flight mass spectrometry. J Biol. Chem. 271: 23222.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Springer Science+Business Media New York

About this chapter

Cite this chapter

Sporns, P., Abell, D.C. (1998). MALDI-MS for Food Analysis. In: Tunick, M.H., Palumbo, S.A., Fratamico, P.M. (eds) New Techniques in the Analysis of Foods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5995-2_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-5995-2_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4419-3307-2

  • Online ISBN: 978-1-4757-5995-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics