Abstract
It is well recognized that neutralization of endotoxin (lipopolysaccharide, LPS) by means of antibodies, could be of value in reducing the high mortality due to Gram-negative sepsis and septic shock. Of particular interest would be the availability of a single antiserum (or: monoclonal antibody), effective against the three bacterial species which are most often implicated, i.e., Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. A polyclonal antiserum, raised against a rough bacterial mutant of E. coli (strain J5) was found to have such cross-protective abilities, and to significantly reduce mortality in human septic shock (21). An intrinsic problem in the use of polyclonal antisera is the difficulty in knowing to which epitope(s) cross-protective antibodies is (are) directed. To answer this question monoclonal antibodies (mAbs) to the (LPS) core (and: lipid A) region have been prepared and tested for protection. The philosophy is that, since the LPS core region and the toxic lipid A part of the major Gram-negative species contain common structural elements, this core-lipid A part would be the ideal candidate for evoking cross-reactive monoclonal antibodies with LPS-neutralizing abilities. Indeed, the preparation of such cross-protective mAbs has been reported by at least two research groups (8, 19).
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Appelmelk, B.J. et al. (1990). Further Characterization of Monoclonal Antibodies to Lipopolysaccharide of Salmonella Minnesota Strain R595. In: Friedman, H., Klein, T.W., Nakano, M., Nowotny, A. (eds) Endotoxin. Advances in Experimental Medicine and Biology, vol 256. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5140-6_27
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DOI: https://doi.org/10.1007/978-1-4757-5140-6_27
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