Abstract
Two alloreactive H-2 (class I) specific cytotoxic IgM monoclonal antibodies (McAbs), B6-1 and B6-2, were isolated from C57B1/6 (B6) mice after injections of syngeneic Sendai virus-coated (SV+) spleen cells. McAb B6-I recognizes the public H-2 class I specificity H-2.25, shared by the H-2k(Kk) and H-2r haplotypes and cross-reacts with H-2z antigens on SV-infected (SVi) lymphoblasts. McAb B6-2 recognizes the private H-2Kf determinant H-2.26 on normal Sendai virus-negative (SV-) spleen cells but cross-reacts with H-2Kk and H-2Dd antigens on SVi blast cells. A polymorphic reaction pattern, that was dependent on the presence of Sendai virus, as tested on a panel of B10 target cells, was also observed with the cytotoxic immune sera from the mice whose cells were used in fusion experiments. Given the fact that each immune serum is an extensive mixture of anti-H-2 antibodies with different specificities, the isolated McAbs can be seen as illustrative examples which explain the reaction pattern of the immune sera. The recognition of polymorphic determinants on SV-infected, positive cells, is presumably due to a modified exposure of cross-reacting public H-2 class I determinants. Taken together, the lymphocytotoxic McAbs induced by injections of syngeneic SV+ cells recognize neither viral antigen nor self-MHC+X composite determinants, but allogeneic H-2 determinants as presented on normal, SV-coated or SV-infected cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Allen, H., Wraith, d., Pala, P., Askonas B., and Flavell, R.A.: Domain interactions of H-2 class I antigens after cytotoxic T-cell recognition sites. Nature 309: 279–281, 1984
Ashwell, J.D., and Schwartz, R.H.: T-cell recognition of antigen and Ia molecules as ternary complex. Nature 320: 176–179, 1986
Babbit, B.P., Allen, P.M., Matsueda, G., Haber, E., and Unanue, E.R.: Binding of immunogenic peptides to Ia histocompatibility molecules. Nature 317: 357–316, 1985.
Cerny-Provaznik, R., van Mourik, P., Limpens, J., Leupers, T., and Ivanyi, P.: Anti-MHC immunity detected prior intentional alloimmunization. III. Natural autoreactive H-2 specific antibodies. Immunogenetics 21: 461–504, 1985
Froscher, B.G., and Klinman, N.R.: Immunization with SV40-transformed cells yields mainly MHC-restricted monoclonale antibodies. J. Exp. Med. 164: 196–210, 1986.
Gorczynski, R.M., Kennedy, M.J., MacRae, S., Steele, E.J., and Cunningham, A.J.: Restriction of antigen recognition in mouse B lymphocytes by genes mapping within the major histocompatibility complex. J. Immunol. 124: 590–596, 1980
Hodes, R.J., and Singer, A.: MHC restricted recognition by antigen-specific B cells: fact or artefact? Immunology Today 5: 50–54, 1984
Ivanyi, P., van den Berg-Loonen, E., and de Greeve, P.: Individual mice of one inbred strain produce anti-H-2 and anti-HLA antibodies of different specificities. Tissue Antigens 12: 32–38, 1978
Kast, W.M., de Waal, L.P., and Melief, C.J.M.: Thymus dictates major histocompatibility complex (MHC) specificity and immune response gene phenotype of class II MHC-restricted T cells but not class I MHC-restricted T cells. J. Exp. Med. 160: 1752–1766, 1984
Katz, D.H. Skidmore B.J., Katz, L.R., and Bogowitz C.A.: Adaptive differentation of murine lymphocytes. I. Both T and B lymphocytes differentiating in F1’ parental chimeras manifest preferential cooperative activity for partner lymphocytes derived from the same parental type corresponding to the chimeric host. J. Exp. Med. 148: 727–745, 1978
Kievits, F., Rocca, A., Opolski, A., Limpens, J., Leupers, T., Kloosterman, T., Boerenkamp, W.P., Pla, M., and Ivanyi, P.: Induction of H-2 specific antibodies by injections of syngeneic Sendai virus-coated cells. Eur. J. Immunol. 17: 27–35, 1987
Van Leeuwen, A.E., Goulmy, A., and van Rood, J.J.: Major histocompatibility complex restricted antibody reactivity mainly, but not exclusively, directed against cells from male donors. J. Exp. Med. 150: 1075–1083, 1979
Matzinger, P.: A one-receptor view of T-cell behaviour. Nature 292: 497–501, 1981
Van Mourik, P., Rivero, R.A., van der Kwast, T.H., Lansdorp, P.M., and Zeijlemaker, W.P.: Density separation of spleen cells increases fusion sequency and yiels of Ig-producing hybridomas. J. Immunol. Methods 68: 45–55, 1984
Nisbet-Brown, E., Singh, B., and Diener, E.: Antigen recognition. V. Requirement for histocompatibility between antigen-presenting cell and B-cell in the response to a thymus-dependent antigen, and lack of allogeneic restriction between T and B cells. J. Exp. Med. 154: 676–687, 1981
Opolski, A., Kievits, F., and Ivanyi, P.: Polymorphic and autoreactive H-2 specific monoclonal antibodies isolated after injections of syngeneic Sendai virus-coated lymphocytes. Immunogenetics 24: 402–408, 1986
Sherman, L.A.: Recognition of conformational determinants on H-2 by cytotoxic T lymphocytes. Nature 297: 511–513, 1982
Singer, A., Hathcock, K.S., and Hodes, R.J.: Cellular and genetic control of antibody responses. V. Helper T-cell recognition of H-2 determinants on accessory cells but not B cells. J. Exp. Med. 149: 1208–1229, 1979
Sprent, J., and Bruce, J.: Lymphoid function in F1’ parent chimeras. Lack of evidence for adaptive differentation of B cells or antigen-presenting cells. J. Exp. Med. 150: 715–720, 1979
Wylie, D.E., Sherman, L.A., and Klinman, N.R.: Participation of the major histocompatibility complex in antibody recognition of viral antigens expressed on infected cells. J. Exp. Med. 155: 403–414, 1982
Zinkernagel, R.M., and Doherty, P.C.: MHC-restricted cytotoxic T cells: Studies on the biological role of polymorphic major transplantation antigens determing T cell restriction-specificity. Adv. Immunol. 27: 51–77, 1979
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer Science+Business Media New York
About this chapter
Cite this chapter
Kievits, F., Opolski, A., Boerenkamp, W.J., Pla, M., Ivanyi, P. (1987). Monoclonal H-2 Class I Specific Antibodies Isolated After Immunization of C57B1/6 Mice with Syngeneic Sendai Virus-Coated Cells. In: David, C.S. (eds) H-2 Antigens. NATO ASI Series, vol 144. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0764-9_63
Download citation
DOI: https://doi.org/10.1007/978-1-4757-0764-9_63
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-0766-3
Online ISBN: 978-1-4757-0764-9
eBook Packages: Springer Book Archive