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Probing Enzyme-Substrate Recognition and Catalytic Mechanism in Cu,Zn Superoxide Dismutase

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Oxygen Radicals in Biology and Medicine

Part of the book series: Basic Life Sciences ((BLSC,volume 49))

Abstract

Superoxide dismutase (SOD) is a ubiquitous enzyme of aerobic organisms that protects against the toxic effects of dioxygen metabolism. SOD catalyzes the dismutation of the superoxide radical, O- 2, to molecular oxygen and hydrogen peroxide through the alternate reduction and oxidation of the active site metal ion (Cu, Mn, or Fe)1:

Cu, Zn SOD occurs almost entirely in eukaryotic cells, while Mn or Fe SODs occur predominantly in prokaryotes and mitochondria. The catalytic rate of SOD is very rapid (2 × 109 M-1sec-1),2 suggesting the evolution of an optimal active site for the recognition and chemical catalysis of the substrate by the enzyme. This remarkably high catalytic rate makes the enzyme a simple model system in which to study the role of electrostatic forces in molecular recognition.

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Authors and Affiliations

  1. Dept. of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California, 92037, USA

    John A. Tainer, Robert A. Hallewell, Victoria A. Roberts, Hans E. Parge & Elizabeth D. Getzoff

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  1. John A. Tainer
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  2. Robert A. Hallewell
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  3. Victoria A. Roberts
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  4. Hans E. Parge
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  5. Elizabeth D. Getzoff
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Editor information

Editors and Affiliations

  1. National Bureau of Standards, Gaithersburg, Maryland, USA

    Michael G. Simic

  2. International Life Sciences Institute, Washington, D.C., USA

    Karen A. Taylor

  3. Univesity of California, San Diego, La Jolla, California, USA

    John F. Ward

  4. Max-Planck-Institut für Strahlenchemie, Mülheim a.d. Ruhr, Federal Republic of Germany

    Clemens von Sonntag

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© 1988 Plenum Press, New York

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Tainer, J.A., Hallewell, R.A., Roberts, V.A., Parge, H.E., Getzoff, E.D. (1988). Probing Enzyme-Substrate Recognition and Catalytic Mechanism in Cu,Zn Superoxide Dismutase. In: Simic, M.G., Taylor, K.A., Ward, J.F., von Sonntag, C. (eds) Oxygen Radicals in Biology and Medicine. Basic Life Sciences, vol 49. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5568-7_100

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  • DOI: https://doi.org/10.1007/978-1-4684-5568-7_100

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5570-0

  • Online ISBN: 978-1-4684-5568-7

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