Abstract
A lipid transfer protein (LTP) was purified from germinated castor bean seeds to homogeneity by column chromatographies. This protein, possessing about 9, 000 of the molecular weight and higher than 10;5 of the isoelectric point, transferred PC, PI, PE, PG, PA, MGDG and DGDG, but not DG and TG, and the transfer activity remained unchanged after 30 min-heating at 90°C. This protein is one of non-specific LTPs as isolated from maize seedlings1 and spinach leaves2. This paper presents the determination of complete amino acid sequence of castor bean non-specific LTP. Based on the amino acid sequence, structural aspect is discussed in relation to the interaction of the protein with the lipid molecule.
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© 1987 Plenum Press, New York
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Yamada, M., Watanabe, S., Takishima, K., Mamiya, G. (1987). Complete Amino Acid Sequence of Non-specific Lipid Transfer Protein from Castor Bean Seeds. In: Stumpf, P.K., Mudd, J.B., Nes, W.D. (eds) The Metabolism, Structure, and Function of Plant Lipids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5263-1_125
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DOI: https://doi.org/10.1007/978-1-4684-5263-1_125
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