Abstract
Expression of recombinant DNA has been achieved in both prokaryotic and eukaryotic cells. This chapter deals with expression of bovine growth hormone recombinant DNA in cultured mammalian cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Andrews, P. (1966) Molecular weights of prolactin and pituitary growth hormones estimated by gel filtration. Nature 209:155–157.
Benoist, C., and P. Chambon (1981) In vivo sequence requirement of the SV40 early promoter region. Nature 290:304–310.
Benoist, C., K. O’Hare, R. Breatnach, and P. Chambon (1980) Ovalbumin gene-sequence of putative control regions. Nucl. Acids Res. 8:127–142.
Bonner, W.M., and R.A. Laskey (1974) A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46:83–88.
Cathala, G., N.L. Eberhardt, N.C. Lan, D.G. Gardner, A. Gutierrez-Hartmann, S.H. Mellon, M. Karin, and J.D. Baxter (1983) Structure and expression of growth hormone-related genes. In Perspective on Genes and the Molecular Biology of Cancer, D.L. Robberson and G.F. Saunders, eds. Raven Press, New York.
Cullen, B.R., P.T. Lomedico, and G. Ju (1984) Transcriptional inter-ference in avian retroviruses-Implications for the promoter insertion model of leukaemogenesis. Nature 307:241–245.
Darnell, J.E., Jr. (1982) Variety in the level of gene control in eucaryotic cells. Nature 297:365–371.
Dellacha, J.M., J.A. Santome, and L. Faiferman (1966) Molecular weight of bovine growth hormone. Experimentia 22:16–17.
Ellis, G.J., E. Marler, H.C. Chen, and A.E. Wilhelmi (1966) Molecular weight of bovine, porcine and human growth hormone by sedimentation equilibrium. Fed. Proc. 25:348.
Goeddel, D.V., H.L. Heyneker, T. Hozumi, R. Arentzen, K. Itakura, D.G. Yansura, J.J. Ross, G. Miozari, R. Crea, and P.H. Seeburg (1979) Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone. Nature 281:544–548.
Greenspan, F.J., C.H. Li, M.E. Simpson, and H.M. Evans (1949) Bioassay of hypophyseol growth hormones: The tibia test. Endocrinology 45: 455–463.
Grosveld, G.C., E. de Boer, C.K. Shewmaker, and R.A. Flavell (1982) DNA sequences necessary for transcription of the rabbit ß-globin gene in vivo. Nature 295:120–126.
Hortin, G., and L. Boime (1980) Inhibition of preprotein processing in ascites tumor lysates by incorporation of a leucine analog. Proc. Natl. Acad. Sci., USA 77:1356–1360.
Ju, G., and A.M. Skala (1980) Nucleotide sequence analysis of the long terminal repeat (LTR) of avian retroviruses: Structural similarities with transposable elements. Cell 22:379–386.
Khoury, G., and P. Gruss (1983) Enhancer elements. Cell 33:313–314.
Kopchick, J.J., and D.W. Stacey (1984) Differences in intracellular DNA ligation after microinjection and transfection. Mol. Cell. Biol.4:240–246.
Kopchick, J.J., R.H. Malavarca, T.J. Livelli, and F.C. Leung (1985) Use of avian retroviral bovine growth hormone DNA recombinants to direct expression of biologically active growth hormone by cultured fibroblasts. DNA 4:23–31.
Kopchick, J.J., W.L. Karshin, and R.B. Arlinghaus (1979) Tryptic peptide analysis of ’gag’ and ’gag-pol’ gene products of Rauscher murine leukemia virus. J. Virol. 30:610–623.
Kozak, M. (1983) Translation of insulin-related polypeptides from messenger RNAs with tandemly reiterated copies of the ribosome binding site. Cell 34:971–978.
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685.
Laimins, L.A., P. Tsichlis, and G. Khoury (1984) Multiple enhancer domains in the 3’ terminus of the Prague strain of Rous sarcoma virus. Nucl. Acids Res. 12:6427–6442.
Laskey, R.A., and A.D. Mills (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur. J. Biochem. 56: 335–341.
Leung, F.C., J.G. Taylor, S.L. Steelman, C.D. Bennett, J.A. Rodkey, R.A. Long, R. Serio, R.M. Weppelman, and G. Olson (1984) Purification and properties of chicken growth hormone and the development of a homologous radioimmunoassay. Gen. Comp. Endocrinol. 56:389–400.
Li, C.H., and L. Ash (1953) The nitrogen terminal end groups of hypophyseal growth hormone. J. Biol. Chem. 203:419–424.
Lingappa, V.R., A. Devillers-Threry, and G. Blobel (1977) Nascent prehormones are intermediates in the biosynthesis of authentic bovine pituitary growth hormone and prolactin. Proc. Natl. Acad. Sci., USA74:2432–2436.
Lopata, M.A., D.W. Cleveland, and B. Soelner-Webb (1984) High levels of transient expression of a chloramphenicol acetyl transferase gene by a DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment. Nucl. Acids Res. 12:5707–5717.
Luciw, P.A., J.M. Bishop, H.E. Varmus, and M.R. Capecchi (1983) Location and function of retroviral and SV40 sequences that enhance biochemical transformation after microinjection of DNA, Cell 33:705–716.
Martin, J.B. (1978) Neural regulation of growth hormone secretion. New Engl. J. Med. 288:1384–1388.
Maxam, A.M., and W. Gilbert (1977) A new method for sequencing DNA. Proc. Natl. Acad. Sci., USA 74:560–564.
Miller, W.L., and N.L. Eberhardt (1983) Structure and evolution of the growth hormone gene family. Endocrine Rev. 4:97–129.
Miller, W.L., D. Coit, J.D. Baxter, and J.A. Martial (1981) Cloning of bovine prolactin cDNA and evolutionary implications of its sequence. DNA 1:37–50.
Miller, W.L., J.A. Martial, and J.D. Baxter (1980) Molecular cloning of DNA complementary to bovine growth hormone mRNA. J. Biol. Chem.255:7521–7524.
Minty, A., and P. Newmark (1980) Gene regulation: New, old and remote controls. Nature 288:210–211.
Niall, H.D., M.L. Hogan, R. Sayer, I.Y. Rosenblum, and F.C. Greenwood (1971) Sequences of pituitary and placental lactogenic and growth hormones: Evolution from a primordial peptide by gene replication. Proc. Natl. Acad. Sci., USA 68:866–869.
Palmiter, R.D., R.L. Brinster, R.E. Hammer, M.E. Taumbauer, M.G. Rosenfeld, N.C. Birnberg, and R.M. Evans (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature 300:611–615.
Pasleau, F., M.J. Tocci, F. Leung, and J.J. Kopchick (1985) Growth hormone gene expression in eukaryotic cells directed by the Rous sar coma viral long terminal repeat or cytomegalovirus immediate early promoter. Gene (in press).
Peel, C.J., D.E. Bauman, R.C. Gorewit, and C.J. Snifton (1981) Effect of exogenous growth hormone on lactational performance in high yielding dairy cows. J. Nutr. 111:1662–1671.
Peel, C.J., T.J. Frank, D.E. Bauman, and R.C. Gorewit (1983) Effect of exogenous growth hormone in early and late lactation on lactation performance of dairy cows. J. Dairy Sci. 66:776–782.
Pellicer, A., D. Robins, B. Wold, R. Sweet, J. Jackson, I. Lowy, J.M. Roberts, G.K. Sim, S. Silverstein, and R. Axel (1980) Altering genotype and phenotype by DNA-mediated gene transfer. Science 209:1414–1422.
Raben, M.S. (1958) Treatment of a pituitary dwarf with human growth hormone. J. Clin. Endocrinol. 18:901–904.
Roberts, J.M., and R. Axel (1982) Gene amplification and gene correction in somatic cells. Cell 29:109–119.
Robins, D.M., I. Paek, P.H. Seeburg, and R. Axel (1982) Regulated expression of human growth hormone genes in mouse cells. Cell 29:623–631.
Schwartz, D.E., R. Tizard, and W. Gilbert (1983) Nucleotide sequence of Rous sarcoma virus. Cell 32:853–869.
Seeburg, P.H., J. Shine, J.A. Martial, J.D. Baxter, and H.M. Goodman (1977) Nucleotide sequence and amplification in bacteria of the structural gene for rat growth hormone. Nature 270:486–494.
Seeburg, P.H., S. Sias, J. Adelman, H.A. De Boer, J. Hayflick, P. Jhurani, D.V. Goeddel, and H.L. Heyneker (1983) Efficient bacterial expression of bovine and porcine growth hormones. DNA 2:37–45.
Skalka, A.M., P.N. Tsichlis, R. Malavarca, B. Cullen, and G. Ju (1983) Viral sequences determining the oncogenicity of avian leukosis viruses. In Oncogenes and Retroviruses: Evaluation of Basic Findings and Clinical Potential, T.E. O’Connor and F. Rauscher, eds. Alan R. Liss, Inc., New York, pp. 105–118.
Sompayrac, L.M., and K.J. Danna (1981) Efficient infection of monkey cells with DNA of simian virus 40. Proc. Natl. Acad. Sci., USA 78: 7575–7578.
Srinivasan, A., R.E. Premkumar, C.Y. Dunn, and S.A. Aaronson (1984) Molecular dissection of transcriptional control elements within the long terminal repeat of the retrovirus. Science 223:286–289.
Stenberg, R.M., D.R. Thomsen, and M.F. Stinksi (1984) Structural analysis of the major immediate early gene in human cytomegalovirus. J. Virol. 49:190–199.
Stinski, M.F., D.R. Thomsen, R.M. Stenberg, and L.C. Goldstein (1983) Organization and expression of the immediate early genes of human cytomegalovirus. J. Virol. 46:1–14.
Swislock, N.I., M. Sonnenber, and N. Yamasaki (1970) In vitro metabolic effects of bovine growth hormone fragment in adipose tissue. Endocrinology 87:900–904.
Temin, H.M. (1982) Function of the retrovirus long terminal repeat. Cell 28:3–5.
Thomsen, D.T., R.M. Stenberg, W.F. Goins, and M.F. Stinski (1984) Promoter-regulatory region of the major immediate early gene of cytomegalovirus. Proc. Natl. Acad. Sci., USA 81:659–663.
Wathen, M.W., and M.F. Stinski (1982) Temporal patterns of human cytomegalovirus transcript: Mapping the viral RNAs synthesized at immediate early, early and late times after infection. J. Virol. 4:462–477.
Walter, P., R. Gilmore, and G. Blobel (1984) Protein translocation across the endoplasmic reticulum. Cell 38:5–8.
Weiher, H., M. Konig, and P. Gruss (1983) Multiple point mutations af-fecting the simian virus 40 enhancer. Science 219:626–631.
Wigler, M., S. Silverstein, L.S. Lee, A. Pellicer, Y.C. Cheng, and R. Axel (1977) Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell 11:223–232.
Wold, B., M. Wigler, E. Lacy, T. Maniatis, S. Silverstein, and R. Axel (1979) Introduction and expression of a rabbit ß-globin gene in mouse fibroblasts. Proc. Natl. Acad. Sci., USA 76:5684–5688.
Woychick, R.P., S.A. Camper, R.H. Lyons, S. Horowitz, E.C. Goodwin, and F.M. Rottman (1982) Cloning and nucleotide sequencing of the bovine growth hormone gene. Nucl. Acids Res. 10:7197–7210.
Yaniv, M. (1982) Enhancing elements for activation of eucaryotic promoters. Nature 297:17–18.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Kopchick, J.J., Pasleau, F., Leung, F.C. (1986). Expression of the Bovine Growth Hormone Gene in Cultured Rodent Cells. In: Evans, J.W., Hollaender, A., Wilson, C.M. (eds) Genetic Engineering of Animals. Basic Life Sciences. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5110-8_5
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5110-8_5
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5112-2
Online ISBN: 978-1-4684-5110-8
eBook Packages: Springer Book Archive