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Human—Human Hybridoma Technology

Five Years of Technical Improvements, and Its Application in Cancer Biology

  • Chapter
Human Hybridomas and Monoclonal Antibodies

Abstract

Cloning and immortalization of antibody-producing human B lymphocytes are required to establish monoclonal cell lines that secrete human immunoglobulin of predefined specificity. Such cultures may be obtained either (1) by transformation of normal B lymphocytes, e.g., by virus, or (2) by somatic cell hybridization of normal B lymphocytes with malignant cells, resulting in cell hybrids that have preserved the secretion of specific antibody of the B lymphocyte and the growth properties of the malignant cells. The former method has been used almost exclusively with Epstein—Barr virus (EBV) as the transforming agent, and some human monoclonal antibodies with interesting specificity have been obtained by this method (Steinitz et al., 1979; Zurawski et al., 1978; Irie et al.,1982; Watson et al., 1983; Rosen et al.,1983).

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Authors and Affiliations

  1. Cancer Biology Laboratory, State University Hospital (Rigshospitalet), DK-2100, Copenhagen, Denmark

    Lennart Olsson & Peter Brams

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  1. Lennart Olsson
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Editors and Affiliations

  1. Department of Pathology, Stanford University School of Medicine, Stanford University Medical Center, 94305, Stanford, California, USA

    Edgar G. Engleman  & Steven K. H. Foung  & 

  2. Cetus Immune Research Laboratories, 94303, Palo Alto, California, USA

    James W. Larrick  & Andrew A. Raubitschek  & 

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Olsson, L., Brams, P. (1985). Human—Human Hybridoma Technology. In: Engleman, E.G., Foung, S.K.H., Larrick, J.W., Raubitschek, A.A. (eds) Human Hybridomas and Monoclonal Antibodies. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4949-5_13

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