Abstract
Prostaglandin E2 (PGE2) isomerase and prostacyclin (PGI2) synthetase are the major endoperoxide (PGH2)-metabolizing enzymes present in microsomes isolated from vascular tissue. The PGE2 isomerase activity specifically requires the addition of GSH (Ogino et al., 1977). Prostacyclin has potent vasodilator and platelet antiag-gregatory activity (Vane, 1983). Therefore, modulation of PGI2 synthetase activity is of prime physiological importance in the regulation of blood vessel function. Reducing agents such as GSH have been reported to augment prostaglandin production by protecting the cyclooxygenase enzyme system (arachidonic acid→ PGH2) from self-catalyzed deactivation (Egan et al, 1976). However, little evidence exists for the effects of these agents on augmenting prostacyclin synthetase activity (McNamara et al.1984). We report the concentration-dependent modulation of the formation of 6-keto-PGF1α, the stable breakdown product of PGI2, by GSH and dithiothreitol (DTT) and unmasking of PGE2 isomerase in microsomes isolated from bovine coronary artery.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Beetens, J. R., Claeys, M., and Herman, A. G., 1983, Vitamin C increases the formation of prostacyclin by aortic rings from various species and neutralizes the inhibitory effect of the 15-hydroxy-arach-idonic acid, Br. J. Pharmacol. 80:249–254.
Bunting, S., Moncada, S., and Vane, J.R., 1983, The prostacyclin-thromboxane A2 balance: Patho-physiological and therapeutic implications, Br. Med. Bull. 39:271–276.
Carrier, R., Cragoe, E.J., Ethier, D., Ford-Hutchinson, A.W., Girard, Y., Hall, R.A., Hamel, P., Rokach, J., Share, N.N., Stone, C.A., and Yusko, P., 1984, Studies on L-640,035: A novel antagonist of contractile prostanoids in the lung, Br. J. Pharmacol. 82:389–395.
Carstein, M.E., and Miller, J.D., 1977, Effects of prostaglandins and oxytocin on calcium release from a uterine microsomal fraction, J. Biol. Chem. 252:1576–1581.
Egan, R.W., Paxton, J., and Kuehl, F.A., Jr., 1976, Mechanism for irreversible self-deactivation of prostaglandin synthetase, J. Biol. Chem. 251:7329–7335.
Fantone, J.C, and Kinnes, D.A., 1983, Prostaglandin E1 and prostaglandin I2 modulation of Superoxide production by human neutrophils, Biochem. Biophys. Res. Commun. 113:506–512.
Farooqui, M.Y.H., and Ahmed, A., 1984, Circadian periodicity of tissue glutathione and its relationship with lipid peroxidation in rats, Life Sci. 34:2413–2418.
Gerritsen, M.E., and Printz, M.P., 1981, Sites of prostaglandin synthesis in the bovine heart and isolated bovine coronary microvessels, Circ. Res. 49:1152–1163.
Greenberg, S., 1981, Effect of prostacyclin and 9,11-epoxymethanoprostaglandin H2 on calcium and magnesium fluxes and tension development in canine intralobar pulmonary arteries and veins, J. Pharmacol. Exp. Ther. 219:326–337.
Hyman, A.L., Kadowitz, P.J., Lands, W.E.M., Crawford, C.G., Fried, J., and Barton, J., 1978, Coronary vasodilator activity of 13,14-dehydroprostacyclin methyl ester: Comparison with prostacyclin and other prostanoids, Proc. Natl. Acad. Sci. U.S.A. 75:3522–3526.
Kerstein, M.D., Saroyan, M., McMullen-Laird, M., Hyman, A.L., Kadowitz, P.J., and McNamara, D.B., 1983, Metabolism of prostaglandins in human saphenous vein, J. Surg. Res. 35:91–100.
Lefer, A.M., Ogletree, M.S., Smith, J.B., Silver, M.J., Nicolaou, K.C, and Gasic, G.P., 1978, Prostacyclin: A potentially valuable agent for preserving myocardial tissue in acute myocardial infarction, Science 200:52–54.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.S., 1951, Protein measurement with folin phenol reagent, J. Biol. Chem. 193:265–275.
McMullen-Laird, M., McNamara, D. B., Kerstein, M. D., Hyman, A. L., and Kadowitz, P. J., 1982, Human lung metabolism of prostaglandin endoperoxide, Circulation 66(11): 166.
McNamara, D.B., Roulet, M.J., Gruetter, C.A., Hyman, A.L., and Kadowitz, P.J., 1980, Correlation of prostaglandin-induced mitochondrial calcium release with contraction in bovine intrapulmonary vein, Prostaglandins 20:311–320.
McNamara, D.B., Hussey, J.L., Kerstein, M.D., Rosenson, R.J., Hyman, A.L., and Kadowitz, P.J., 1984, Modulation of prostacyclin synthetase and unmasking of PGE2 isomerase in bovine coronary arterial microsomes, Biochem. Biophys. Res. Commun. 118:33–39.
Meister, A., and Anderson, M. E., 1983, Glutathione, Annu. Rev. Biochem. 52:711–760.
Needleman, P., 1976, Organic nitrate metabolism, Annu. Rev. Pharmacol. 16:81–93.
Ogino, N., Miyamoto, T., Yamamoto, S., and Hayaishi, O., 1977, Prostaglandin endoperoxide E isomerase from bovine vesicular gland microsomes, a glutathione requiring enzyme, J. Biol. Chem. 252:890–895.
Okuma, M., Takayma, H., and Uchino, H., 1980, Generation of prostacyclin-like substance and lipid peroxidation in vitamin E-deficient rats, Prostaglandins 19:527–536.
Romson, J.L., Hook, B.G., Rigot, V.H., Schork, M.A., Swanson, D.P., and Lucchesi, B.R., 1982, The effect of ibuprofen on accumulation of Indium-lll-labeled platelets and leukocytes in experimental myocardial infarction, Circulation 66:1002–1011.
Schror, K., Grodzinska, L., and Darius, H., 1981, Stimulation of coronary vascular prostacyclin and inhibition of human platelet thromboxane A2 after low dose nitroglycerin, Thromb. Res. 23:59–67.
She, H.S., McNamara, D.B., Spannhake, E.W., Hyman, A.L., and Kadowitz, P.J., 1981, Metabolism of prostaglandin endoperoxide by microsomes from cat lung, Prostaglandins 21:531–541.
Thomas, G., Skrinska, V., Lucas, F., and Butkus, A., 1984, Platelet glutathione and thromboxane synthesis in diabetes mellitus, Fed. Proc. 43:1040.
Vane, J. R., 1983, Adventures and excursions in bioassay: The stepping stones to prostacyclin, Br. J. Pharmacol. 79:821–838.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1985 Springer Science+Business Media New York
About this chapter
Cite this chapter
McNamara, D.B. et al. (1985). Coronary Arterial Prostacyclin Synthetase and Prostaglandin E2 Isomerase Activities. In: Bailey, J.M. (eds) Prostaglandins, Leukotrienes, and Lipoxins. GWUMC Department of Biochemistry Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4946-4_6
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4946-4_6
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4948-8
Online ISBN: 978-1-4684-4946-4
eBook Packages: Springer Book Archive