Abstract
The mitochondrial ATPase complex has been resolved into the soluble F1, having the catalytic site for ATP synthesis, and the hydrophobic membrane-integrated F0, involved in proton translocation (Senior, 1973; Koslov and Skulacher, 1977). The F0 has been studied with the use of oligomycin and dicyclohexylcarbodiimide (DCCD), which inhibit enzyme activity of the complex by acting on a component of F0, (Bulos and Racker, 1968; Roberton et al., 1968; Sebald et al., 1976, 1979; Kiehl and Hatefi, 1980).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Anderson, S., Bankier, A. T., Barrell, B. G., de Bruijn, M. H. L., Coulson, A. R., Drouin, J., Eperon, I. C., Nierlich, D. P., Roe, B. A., Sanger, F., Schreier, P. H., Smith, A. J. H., Staden, R., and Young, I. G., 1981, Sequence and organization of the human mitochondrial genome, Nature 290:457–465.
Avner, P. R., Coen, D., Dujon, B., and Slonimski, P. P., 1973, Mitochondrial genetics. IV. Allelism and mapping studies of oligomycin resistant mutants in S. cerevisiae, Mol. Gen. Genet. 125:9–52.
Breen, G. A. M., and Scheffler, I. E., 1980, Cytoplasmic inheritance of oligomycin resistance in Chinese hamster ovary cells, J. Cell. Biol. 86:723–729.
Bulos, B., and Racker, E., 1968, Partial resolution of the enzymes catalyzing oxidative phosphorylation, J. Biol. Chem. 243:3891–3900.
Bunn, C. L., Wallace, D. C., and Eisenstadt, J. M., 1977, Cytoplasmic inheritance of chloramphenicol resistance in mouse tissue culture cells, Proc. Natl. Acad. Sci. USA 71:1681–1685.
Cattel, K. J., Knight, I. G., Lindop, G. R., and Beechey, R. B., 1970, The isolation of dicyclohexyl-carbodiimide binding proteins from mitochondrial membranes, Biochem. J. 117:1011–1013.
Criddle, R. S., Arulanadan, C., Edwards, T., Johnson, R., Scharf, S., and Enns, R., 1979, Investigation of the oligomycin binding protein in yeast mitochondrial ATPase, in: Genetics and Biogenesis of Chloroplasts and Mitochondria (T. Bucher, ed.), Elsevier North-Holland, Amsterdam, pp. 151–157.
Edwards, D. L., and Urger, B. W., 1978, Nuclear mutations conferring oligomycin resistance in Neurospora crassa, J. Biol. Chem. 253:4254–4258.
Hensgens, L. A. M., Grivell, L. A., Borst, P., and Bos, J. L., 1979, Nucleotide sequence of the mitochondrial structural gene for subunit 9 of yeast ATPase complex, Proc. Natl. Acad. Sci. USA 765:1663–1667.
Howell, N., and Sager, R., 1979, Cytoplasmic genetics of mammalian cells: Conditional sensitivity to mitochondrial inhibitors and isolation of new mutant phenotypes, Somat. Cell Genet. 5:833–845.
Jackl, G., and Sebald, W., 1975, Identification of two products of mitochondrial protein synthesis associated with mitochondrial adenosine triphosphatase from Neurospora Crassa, Eur. J. Biochem. 54:97–106.
Kiehl, R., and Hatefi, Y., 1980, Interaction of [14C] dicyclohexylcarbodiimide with complex V (mitochondrial adenosine triphosphate synthetase complex), Biochemistry 19:541–548.
Koslov, I. A., and Skulacher, V. P., 1977, H ± Adenosine triphosphate and membrane energy coupling, Biochim. Biophys. Acta 463:29–89.
Kuhns, M. C., and Eisenstadt, J. M., 1979, Oligomycin-resistant mitochondrial ATPase from mouse fibroblasts, Somat. Cell Genet. 5:821–832.
Kuhns, M. C., and Eisenstadt, J. M., 1981a, Nuclear inheritance of oligomycin resistance in mouse L cells, Somat. Cell Genet., 7:737–750.
Kuhns, M. C., and Eisenstadt, J. M., 1981b, A modified DCCD-binding protein in mouse fibroblast mutants with decreased sensitivity to oligomycin and dicychohexylcarbodiimide, Fed. Proc. 40:1277a.
Kuhns, M. C., and Eisenstadt, J. M., 1982, The dicyclohexylcarbodiimide-binding protein of the mitochondrial ATPase from mouse L cells, submitted.
Lagarde, A. E., and Siminovitch, L., 1979, Studies on Chinese hamster ovary mutants showing multiple cross-reference to oxidative phosphorylation inhibitors, Somat. Cell Genet. 5:847–871.
Lichtor, T., and Getz, G. S., 1978, Cytoplasmic inheritance of rutamycin resistance in mouse fibroblasts, Proc. Natl. Acad. Sci. USA 75:323–328.
Macino, G., Scazzocchio, C., Waring, R. B., McPhail Berks, M., and Davies, R. W., 1980, Conservation and rearrangement of mitochondrial structural gene sequences, Nature 288:404–406.
Pullman, M. E., Penefsky, H. S., Datta, A., and Racker, E., 1960, Partial resolution of the enzymes catalyzing oxidative phosphorylation, J. Biol. Chem. 235:3322–3329.
Roberton, A. M., Holloway, C. T., Knight, I. G., and Beechey, R. B., 1968, Biochem. J. 108:444–456.
Rowlands, R. T., and Turner, G., 1973, Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans, Mol. Gen. Genet. 126:201–216.
Sebald, W., and Wachter, E., 1978, Amino acid sequence of the putative protophore of the energy-transducing ATPase complex, in: Energy Conservation in Biological Membranes (G. Schäfer, and M. Klingenberg, eds), Springer-Verlag, New York, pp. 228–236.
Sebald, W., Graft, T., and Wild, G., 1976, Cytoplasmic synthesis of the dicyclohexylcarbodiimidebinding protein in Meurospora Crassa, in: Genetics and Biogenesis of Chloroplasts and Mitochondria (T. Bucher, ed.), Elsevier North-Holland, Amsterdam, pp. 167–174.
Sebald, W., Wachter, E., and Tzagoloff, A., 1979, Identification of amino acid substitutions in the dicyclohexylcarbodiimide-binding subunit of the mitochondrial ATPase complex from oligomycin-resistant mutants of Saccharomyces cerevisiae, Eur. J. Biochem. 100:559–607.
Senior, A. E., 1973, The structure of mitochondrial ATPase, Biochim. Biophys. Acta 301:249–277.
Sierra, M. F., and Tzagoloff, A., 1973, Assembly of the mitochondrial membrane system. Purification of a mitochondrial product of the ATPase, Proc. Natl. Acad. Sci. USA 70:3155–3159.
Turner, G., Imam, G., and Küntzel, H., 1979, Mitochondrial ATPase complex of Aspergillus nidulans and the dicyclohexylcarbodiimide-binding protein, Eur. J. Biochem. 97:565–571.
Tzagoloff, A., Akai, A., and Needleman, R. B., 1975, Characterization of nuclear mutants of Saccharomyces cerevisiae with defects in mitochondrial ATPase and respiratory enzymes, J. Biol. Chem. 250:8228–8235.
Wigler, M. H., and Weinstein, I. B., 1975, A preparative method for obtaining enucleated mammalian cells, Biochem. Biophys. Res. Commun. 63:669–674.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Plenum Press, New York
About this chapter
Cite this chapter
Eisenstadt, J.M., Kuhns, M.C. (1982). Inheritance of Oligomycin Resistance in Tissue Culture Cells. In: Shay, J.W. (eds) Techniques in Somatic Cell Genetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4271-7_9
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4271-7_9
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4273-1
Online ISBN: 978-1-4684-4271-7
eBook Packages: Springer Book Archive