Abstract
A new exonuclease of Escherichia coli K12, exonuclease VII, has been purified 1700-fold and characterized. The enzyme is specific for single-stranded DNA and can initiate hydrolysis at both 5′ and 3′ termini. It is also capable of thyminedimer excision in vitro. The limit products of the reaction are oligonucleotides, predominantly in the range of tetramers to dodecamers. DNA is hydrolyzed by the enzyme in a processive fashion. Mutants of E. coli have been isolated having reduced levels of exonuclease VII activity in crude extracts. Mapping studies place the exonuclease VII locus between 45 and 56 minutes on the E. coli K12 linkage map.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Chase, J. W. and Richardson, C. C. (1974a). J. Biol. Chem. 249, 4545.
Chase, J. W. and Richardson, C. C. (1974b). J. Biol. Chem. 249, 4553.
Cooper, P. K. and Hanawalt, P. C. (1972). J. Mol. Biol. 67, 1.
Frenkel, G. D. and Richardson, C. C. (1971). J. Biol. Chem. 246, 4839.
Kaplan, J. C., Kushner, S. R. and Grossman, L. (1971). Biochemistry 10, 3315.
Kushner, S. R., Kaplan, J. C., Ono, H. and Grossman, L. (1971). Biochemistry 10, 3325.
Milcarek, C. and Weiss, B. (1972). J. Mol. BioL. 68, 303.
Rupp, W. D. and Howard-Flanders, P. (1968). J. Mol. BioL. 31, 291.
Signer, E. (1971). In The Bacteriophage Lambda (Hershey, A. D., ed.), p. 139. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Taylor, A. L. and Trotter, C. D. (1972). Bacteriol. Rev. 36, 504.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1975 Plenum Press, New York
About this chapter
Cite this chapter
Chase, J.W., Richardson, C.C. (1975). Exonuclease VII of Escherichia coli . In: Hanawalt, P.C., Setlow, R.B. (eds) Molecular Mechanisms for Repair of DNA. Basic Life Sciences. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2895-7_30
Download citation
DOI: https://doi.org/10.1007/978-1-4684-2895-7_30
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-2897-1
Online ISBN: 978-1-4684-2895-7
eBook Packages: Springer Book Archive