Abstract
Purification of the photoreactivating enzyme from Escherichia coli depends on three major factors: (1) development of a suitable assay, (2) selection of a source with high enzyme levels, and (3) development of a gentle, specific purification procedure. The preparation of purified enzyme is useful both in study of DNA repair enzymes and in the analysis of the role of cyclobutyl pyrimidine dimers in the induction of biological damage by ultraviolet light.
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© 1975 Plenum Press, New York
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Sutherland, B.M. (1975). Purifying the Escherichia coli Photoreactivating Enzyme. In: Hanawalt, P.C., Setlow, R.B. (eds) Molecular Mechanisms for Repair of DNA. Basic Life Sciences. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2895-7_13
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DOI: https://doi.org/10.1007/978-1-4684-2895-7_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-2897-1
Online ISBN: 978-1-4684-2895-7
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