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Changes of Purine Metabolism During Differentiation of Rat Heart Myoblasts

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Purine and Pyrimidine Metabolism in Man V

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 195B))

Abstract

During the last decade studies of cultured cells have become a tool for biochemists and physiologists. Investigations in cultivated cardiac and skeletal muscle cells have proven to be good models to study metabolism, drug effects electrophysiological behaviour, and hormone responses of the heart and muscle tissue (1-4).Most of the cardiac cells obtained from neonatal rat hearts contain a mixed population of different cells (myocytes, fibroblasts, and endothelial cells)(4).Therefore it seemed reasonable to investigate a homogenous clonal cell line.We se lected the cell line H9c2 (2-1) derived from embryonic rat heart tissue.It has been reported that this cell line developed properties of skeletal muscle during differentiation in selective medium(5).The aim of this study was to characterize this clonal cell line with respect to purine metabolism and ultrastructural features.Furthermore it should be investigated if differentiation from embryonic heart cells (myoblasts) towards differentiated cells(myocytes)using selective cultivation conditions(5,6)can result in changes of purine metabolism.)

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Authors and Affiliations

  1. 2nd Department of Surgery, Division of Clinical Biochemistry, University of Vienna, Vienna, Austria

    Mathias M. Müller, Helmut RumpoId, Gerhard Schopf & Peter Zilla

Authors
  1. Mathias M. Müller
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  2. Helmut RumpoId
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  3. Gerhard Schopf
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  4. Peter Zilla
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Editor information

Editors and Affiliations

  1. University of California, San Diego La Jolla, California, USA

    W. L. Nyhan

  2. Scripps Clinic and Research Foundation, La Jolla, California, USA

    L. F. Thompson

  3. M. R. C. Clinical Research Center, Harrow, UK

    R. W. E. Watts

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© 1986 Plenum Press, New York

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Müller, M.M., RumpoId, H., Schopf, G., Zilla, P. (1986). Changes of Purine Metabolism During Differentiation of Rat Heart Myoblasts. In: Nyhan, W.L., Thompson, L.F., Watts, R.W.E. (eds) Purine and Pyrimidine Metabolism in Man V. Advances in Experimental Medicine and Biology, vol 195B. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-1248-2_74

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  • DOI: https://doi.org/10.1007/978-1-4684-1248-2_74

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4684-1250-5

  • Online ISBN: 978-1-4684-1248-2

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