Abstract
The purpose of this paper is to reappraise the sequential barriers to O2 transport from red cells to mitochondria in light of a more accurate model of intracapillary O2 transport, and measurements of myoglobin (Mb) saturation in subcellular volumes. We find that transcapillary gradients are larger and tissue gradients smaller than predicted by existing models of O2 diffusion. During exercise the principal “resistance” to O2 transport resides in the capillary and extracellular space; the limiting variables are rate of O2 release and red cell transit time. Mb plays a major role in overcoming “resistance” at the capillary by buffering Po2 well below capillary Po2 (Pcapo2). These findings are incompatible with classical concepts of O2 delivery to red muscle. Alternatives to Kroghian tinking are proposed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Buchtal, F., and Schmalbuch, H., 1980, Motor unit of mammalian muscle, Physiol. Rev., 60: 90.
Chance, B., Oshino, N., Sugano, T., and Mayevsky, A., 1973, Basic principles of tissue oxygen determination from mitochondrial signals, Adv. Exp. Med. Biol., 37a: 277.
Clark, A., and Clark, P., The capture of chemical reations in tissue by freezing, Math. Biosci., in preparation.
Cole, R.P., 1982, Myoglobin function in exercising skeletal muscle, Science, 216: 523.
Federspiel, W.J., Clark, A. Jr., and Cokelet, G.R., 1982, Oxygen delivery: the view from the red cell, Microvasc. Res., 23: 251.
Federspiel, W.J., and Sarelius, I.H., Role of red cell spacing on the intracapillary transport of oxygen, Microvasc. Res., in preparation.
Fletcher, J.E., 1980, On facilitated oxygen diffusion in muscle tissues, Biophys. J., 29: 437.
Gayeski, T., 1981, A cryogenic microspectrophotometric method for measuring myoglobin saturation in subcellular volumes; Application to resting dog gracilis muscle. Ph.D. Dissertation, University of Rochester, Rochester, N.Y.
Gayeski, T.E.J., and Honig, C.R., 1978, Myoglobin saturation and calculated Po2 in single cells of resting gracilis muscles, Adv. Exp. Med. Biol., 94: 77.
Gayeski, T.E.J., and Honig, C.R., 1982, Direct measurement of intracellular O2 gradients: role of convection and myoglobin, Adv. Exp. Med. Physiol., in press.
Grunewald, W.A., and Sowa, W., 1977, Capillary structures and O2 supply to tissue, Rev. Physiol. Biochem. Pharmacol., 77: 149.
Heliums, J.D., 1977, The resistance to oxygen transport in the capillaries relative to that in the surrounding tissue, Microvasc. Res., 13: 131.
Honig, CR., 1981, “Modern Cardiovascular Physiology”, Little Brown Co., Boston, pp. 223–227.
Honig, CR., and Odoroff, CL., 1981, Calculated dispersion of capillary transit times: significance for oxygen exchange, Am. J. Physiol., 240: H199.
Honig, CR., Odoroff, CL., and Frierson, J.L., 1982, Active and passive capillary control in a red muscle at rest and in exercise, Am. J. Physiol., 243: H196.
Huxley, V.H., and Kutchai, H., 1981, The effect of the red cell membrane and a diffusion boundary layer on the rate of oxygen uptake by human erythrocytes, J. Physiol., 316: 75.
Klitzman, B., and Duling, B.R., 1979, Microvascular hematocrit and red cell flow in resting and contracting striated muscle, Am. J. Physiol., 237: H481.
Kreuzer, F., and Yahr, W.Z., 1960, Influence of red cell membrane on diffusion of oxygen, J. Appl. Physiol., 15: 1117.
Kutchai, H., 1970, Numerical study of oxygen uptake by layers of hemoglobin solution, Respir. Physiol., 10: 273.
Moll, W., 1969, The influence of hemoglobin diffusion on oxygen uptake and release by red cells, Respir. Physiol., 6: 1.
Popel, A.S., and Gross, J.F., 1979, Analysis of oxygen diffusion from arteriolar networks, Am. J. Physiol., 237: H681–H689.
Rose, C.P., and Goresky, CA., 1982, Barrier-limited transport of oxygen in the coronary circulation, Fed. Proc, 41: 1252.
Sarelius, I.H., and Duling, B.R., Direct measurement of microvascular hematocrit, red cell flux and transit time, Am. J. Physiol., in press.
Sheth, B.V., and Heliums, J.D., 1980, Transient oxygen transport in hemoglobin layers under conditions of the microcirculation, Ann. B iomed. Eng., 8: 183.
Sinha, A.K., 1969, Oxygen uptake and release by red cells through capillary wall and plasma layer (thesis), Univ. of California, San Francisco, CA.
Tamura, M., Oshino, N., Chance, B., and Silver, I.A., 1978, Optical measurements of intracellular oxygen concentration of rat heart in vitro, Arch. Biochem. Biophys., 191: 8.
Thews, G., 1959, Untersuchung der Sauerstoffaufnahme und-abgäbe sehr dünner Blutlamellen, Pflügers Arch. Ges. Physiol., 268: 308.
Villadsen, J., and Sorensen, J.P., 1969, Solution of parabolic partial differential equations by a double collection method, Chem. Eng. Sci., 24: 1337.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Honig, C.R., Gayeski, T.E.J., Federspiel, W., Clark, A., Clark, P. (1984). Muscle O2 Gradients from Hemoglobin to Cytochrome: New Concepts, New Complexities. In: Lübbers, D.W., Acker, H., Leniger-Follert, E., Goldstrick, T.K. (eds) Oxygen Transport to Tissue-V. Advances in Experimental Medicine and Biology, vol 169. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1188-1_2
Download citation
DOI: https://doi.org/10.1007/978-1-4684-1188-1_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1190-4
Online ISBN: 978-1-4684-1188-1
eBook Packages: Springer Book Archive