Abstract
Before beginning this section, I want to recommend the book Fixation for Electron Microscopy (Hayat, 1981), which is an invaluable source and contains information concerning all aspects of biological specimen preparation in much greater depth than can be provided in this text.
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References
Aldrich, H.C., Biemborn, D.B., and Schonheit, P. 1987. Creation of artifactual internal membranes during fixation of Methanobacterium thermoautotrophicum. Can. J. Microbiol. 33:844.
Beckman, H.-J., and Dierichs, R. 1982. Lipid extracting properties of 2,2-dimethoxypropane as revealed by electron microscopy and thin layer chromatography. Histochemistry 76:407.
Carson, F., Martin, J.H., and Lynn, J.A. 1973. Formalin fixation for electron microscopy: A réévaluation. Am. J. Clin. Pathol. 59:365.
Causton, B.E. 1981. Resins: Toxicity, hazards, and safe handling.Proc. Ry. Microsc. Soc. 16:265.
Coulter, H.D. 1967. Rapid and improved methods for embedding biological tissues in Epon-812 and Araldite 502. J. Ultrastruct. Res. 20:346.
Ghadially, F.N. 1975. Ultrastructural pathology of the cell. Butterworths, London.
Ghadially, F.N. 1985. Diagnostic electron microscopy of tumors, 2nd ed. Butterworths, London.
Gilkey, J.C., and Staehelin, L.A. 1986. Advances in ultrarapid freezing for the preservation of cellular ultrastructure. J. Electron Microsc. Tech. 3:177.
Glauert, A.M. 1975. Fixation, dehydration and embedding of biological specimens. Elsevier North- Holland, New York.
Glauert, A.M., Rogers, G.E., and Glauert, R.H. 1956. A new embedding medium for electron microscopy. Nature 178:803.
Hanstede, J.G., and Gerrits, P.O. 1983. The effects of embedding in water-soluble plastics on the final dimensions of liver sections.J. Microsc. 131:79.
Hayat, M.A. 1981. Fixation for electron microscopy. Academic Press, New York.
Hayat, M.A. 1989. Principles and techniques of electron microscopy: Biological applications, 3rd ed. CRC Press, Boca Raton, FL.
Heckman, C.A., and Barrnett, R.J. 1973. GACH: A water-miscible, lipid-retaining embedding polymer for electron microscopy. J. Ultrastruct. Res. 42:156.
Hopwood, D. 1967. Some aspects of fixation with glutaraldehyde: A biochemical and histochemical comparison of the effects of formaldehyde and glutaraldehyde fixation on various enzymes and glycogen with a note on penetration of glutaraldehyde into liver. J. Anat. 101:83.
Hopwood, D. 1972. Theoretical and practical aspects of glutaraldehyde fixation. In: Fixation in histochemistry, P.J. Stoward (ed). Chapman and Hall, London.
Karnovsky, M.J. 1965. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27:137A.
Kellenberger, E., Schwab, W., and Ryter, A. 1956. L’utilisation d’un copolymère des polyesters comme materiel d’inclusion en ultramicrotomie. Experientia 12:421.
Leong, A.S.-Y., Daymon, M.E., and Milios, J. 1985. Microwave irradiation as a form of fixation for light and electron microscopy. J. Pathol. 146:313.
Leong, A.S.-Y., and Gove, D.W. 1990. Microwave techniques for tissue fixation, processing and staining. Electron Microsc. Soc. Amer. Bull. 20:61.
Login, G.R., Dwyer, B.K., and Dvorak, A.M. 1990. Rapid primary microwave-osmium fixation. I. Preservation of structure for electron microscopy in seconds. J. Histochem. Cytochem. 38:755.
Luft, J.H. 1956. Permanganate—a new fixative for electron microscopy.J. Biophys. Biochem. Cytol. 2:799.
Luft, J.H. 1959. The use of acrolein as a fixative for light and electron microscopy. Anat. Record 133:305.
Luft, J.H. 1961. Improvements in epoxy resin embedding methods. J. Biophys. Biochem. Cytol. 9:409.
Luft, J.H., and Wood, R.L. 1963. The extraction of tissue protein during and after fixation with osmium tetroxide in various buffer systems. J. Cell Biol. 19:46A.
McDowell, E.M., and Trump, B.F. 1976. Histologic fixatives suitable for diagnostic light and electron microscopy. Arch. Pathol. Lab. Med. 100:405.
Millonig, G., and Marinozzi, V. 1968. Fixation and embedding in electron microscopy. In: Advances in optical and electron microscopy, Vol. 2., R. Baser and V.E. Cosslett (eds.), Academic Press, New York.
Mollenhauer, H.H. 1964. Plastic embedding mixtures for use in electron microscopy. Stain Tech. 39:111.
Mollenhauer, H.H. 1986. Surfactants as resin modifiers and their effect on sections. J. Electron Microsc. Tech. 3:217.
Newman, S.B., Borysko, E., and Swerdlo, M. 1949. New sectioning techniques for light and electron microscopy. Science 110:66.
Page, S.G., and Huxley, H.E. 1963. Filament lengths in striated muscle. J. Cell Biol. 19:369.
Palade, G.E. 1952. A study of fixation for electron microscopy. J. Experim. Med. 95:285.
Pease, D.C., and Peterson, R.G. 1972. Polymerizable glutaraldehyde-urea mixtures as polar, water- containing embedding media. J. Ultrastruct. Res. 41:133.
Sabatini, D.D., Miller, F., and Barrnett, R.J. 1963. Cytochemistry and electron microscopy, the preservation of cellular ulstrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17:19.
Salema, R., and Brandao, I. 1973. The use of PIPES buffer in the fixation of plant cells for electron microscopy. J. Submicrosc. Cytol. 5:79.
Smith, R.E., and Farquhar, M.G. 1966. Lysosome function in the regulation of the secretory process in the cells of the anterior pituitary gland. J. Cell Biol. 31:319.
Spurr, A.R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26:31.
Thorpe, J.R., and Harvey, D.M.R. 1979. Optimization and investigation of the use of 2,2- dimethoxypropane as a dehydration agent for plant tissues in transmission electron microscopy. J. Ultrastruct. Res. 68:186.
Tokuyasu, K.T. 1983. Present state of immunocryoultramicrotomy. J. Histochem. Cytochem. 31:164.
Wolosewick, J.J. 1980. The application of polyethylene glycol (PEG) to electron microscopy. J. Cell Biol. 86:675.
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Dykstra, M.J. (1992). Specimen Preparation for Transmission Electron Microscopy. In: Biological Electron Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0010-6_2
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