Abstract
Electron spin resonance spectroscopy has only been used successfully on dry DNA at room temperature or on aqueous DNA at low temperatures. Under these conditions the direct damage mechanism is shown to dominate. The results inicate that electron-loss, which must initially be indiscriminate, rapidly ends up as G·+, which is stable up to ca. 210°K. Electrons are trapped at T, giving T− anions, which are converted into TH in the 130–208°K K range. Above these temperatures, both centres decay without the clear appearance of other intermediate radicals. Arguments are given against the concept that holes and/or electrons are extensively mobile within DNA molecules, and also against the concept that the ionic species studied by ESR spectroscopy recombine to give G and T to a major extent.
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© 1986 Plenum Press, New York
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Cullis, P.M., Symons, M.C.R. (1986). Electron Spin Resonance Studies of the Mechanism of Radiation Damage to DNA. In: Simic, M.G., Grossman, L., Upton, A.C., Bergtold, D.S. (eds) Mechanisms of DNA Damage and Repair. Basic Life Sciences, vol 189. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9462-8_4
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DOI: https://doi.org/10.1007/978-1-4615-9462-8_4
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