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Activation of the NAD Glycohydrolase, NAD:Agmatine and NAD:Gsα ADP-Ribosyltransferase and Auto-ADP-Ribosylation Activities of Choleragen by Guanyl Nucleotide and Soluble Proteins Purified from Bovine Brain

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ADP-Ribose Transfer Reactions

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Abstract

Choleragen (cholera toxin) exerts its effects on animal cells by activating adenylate cyclase, thereby increasing intracellular cAMP content (1). The A1 protein of choleragen, released from the holotoxin by reduction of a single disulfide bond linking the A1 and A2 proteins, catalyzes the mono- ADP-ribosylation of Gsα, a regulatory component of the adenylate cyclase system that is responsible for the GTP-dependent activation of the cyclase catalytic unit. ADP-ribosylation of Gsα apparently increases its sensitivity to GTP and its dissociation from the inhibitory Gβγ complex (1).

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References

  1. Moss, J., Vaughan, M. (1988) Adv Enzymology 61: 303–379

    CAS  Google Scholar 

  2. Enomoto, K., Gill, D.M. (1980) J Biol Chem 255: 1252–1258

    PubMed  CAS  Google Scholar 

  3. Nakaya, S., Moss, J., Vaughan, M. (1980) Biochemistry 19: 4871–4874

    Article  PubMed  CAS  Google Scholar 

  4. Le Vine III, H., Cuatrecasas, P. (1981) Biochim Biophys Acta 672: 248–261

    Article  PubMed  Google Scholar 

  5. Schleifer, L.S., Kahn, R.A., Hanski, E., Northup, J.K., Stemweis, P.C., Gilman, A.G. (1982) J Biol Chem 257: 20–23

    PubMed  CAS  Google Scholar 

  6. Gill, D.M., Meren, R. (1983) J Biol Chem 258: 11908–11914

    PubMed  CAS  Google Scholar 

  7. Kahn, R.A., Gilman, A.G. (1984) J Biol Chem 259: 6228–6234

    PubMed  CAS  Google Scholar 

  8. Kahn, R.A., Gilman, A.G. (1984) J Biol Chem 259: 6235–6240

    PubMed  CAS  Google Scholar 

  9. Kahn, R.A., Gilman, A.G. (1986) J Biol Chem 261: 7906–7911

    PubMed  CAS  Google Scholar 

  10. Tsai, S.-C., Noda, M., Adamik, R., Moss, J., Vaughan, M. (1987) Proc Natl Acad Sci USA 84: 5139–5142

    Article  PubMed  CAS  Google Scholar 

  11. Moss, J., Stanley, S.J., Watkins, P.A. (1980) J Biol Chem 255: 5838–5840

    PubMed  CAS  Google Scholar 

  12. Moss, J., Oppenheimer, N.J., West Jr., R.E., Stanley, S.J. (1986) Biochemistry 25: 5408–5414

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Laboratory of Cellular Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, 20892, USA

    Masatoshi Noda, Su-Chen Tsai, Ronald Adamik, Patrick P. Chang, Barbara C. Kunz, Joel Moss & Martha Vaughan

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  1. Masatoshi Noda
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  2. Su-Chen Tsai
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  3. Ronald Adamik
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  4. Patrick P. Chang
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  5. Barbara C. Kunz
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  6. Joel Moss
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  7. Martha Vaughan
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, Texas College of Osteopathic Medicine, Fort Worth, Texas, 76107-2690, USA

    Myron K. Jacobson

  2. Department of Medicine, Texas College of Osteopathic Medicine, Fort Worth, Texas, 76107-2690, USA

    Elaine L. Jacobson

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© 1989 Springer-Verlag New York Inc.

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Noda, M. et al. (1989). Activation of the NAD Glycohydrolase, NAD:Agmatine and NAD:Gsα ADP-Ribosyltransferase and Auto-ADP-Ribosylation Activities of Choleragen by Guanyl Nucleotide and Soluble Proteins Purified from Bovine Brain. In: Jacobson, M.K., Jacobson, E.L. (eds) ADP-Ribose Transfer Reactions. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-8507-7_86

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  • DOI: https://doi.org/10.1007/978-1-4615-8507-7_86

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4615-8509-1

  • Online ISBN: 978-1-4615-8507-7

  • eBook Packages: Springer Book Archive

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