Abstract
Clostridium pasteurianum is an obligately anaerobic and free-living, nitrogen-fixing organism. It appears to be the first nitrogen-fixing organism isolated, being described by Winogradsky in the mid-1890s (Winogradsky, 1895). Consistent nitrogen-fixing activities using cell-free preparations were first obtained using this organism (Carnahan et al., 1960), which ushered in modern studies on the biochemistry of nitrogen fixation (Burris, 1988). Although the nitro-genase from C. pasteurianum has been extensively studied (see references in Chen et al., 1986), the genetics of the nitrogen fixation system of this organism was not actively investigated when genetic manipulation systems became available in Klebsiella pneumoniae and in other free-living and symbiotic species. The nitrogen-fixation (nif) genes of C. pasteurianum have been studied only in recent years.
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Johnson, J.L., Wang, SZ., Chen, JS. (1993). Organization of the Nitrogen Fixation Genes in Clostridium pasteurianum . In: Sebald, M. (eds) Genetics and Molecular Biology of Anaerobic Bacteria. Brock/Springer Series in Contemporary Bioscience. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-7087-5_26
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DOI: https://doi.org/10.1007/978-1-4615-7087-5_26
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