Abstract
Reports from a number of laboratories have demonstrated that UV-irradiation can cause protein damage in the ocular lens and the inactivation of lens enzymes (Li et al., 1990; Zigman et al., 1991; Rafferty et al., 1993; Hightower, 1995). Glycation-induced damage to structural proteins—hemoglobin and γ-crystalline (Pennington and Harding, 1994), and enzymes—glucose 6-phosphate dehydrogenase (Ganea and Harding, 1995), Cu-Zn-superoxide dismutase (Arai et al., 1987), and glutathione reductase (Blakytny and Harding, 1992), glucokinase (Zahner et al., 1990) has been widely studied. Although, glycation of proteins is thought to play a major role in aging, diabetes, and cataract formation (van Boekel, 1991), the reaction between protein and glucose is considered a slow process. We are interested in the UV-irradiation of lens enzymes in the presence of a high concentration of sugars as a means of elucidating the morphological and physiological sequellae in cataract formation. Here, we report on the enhancement of bovine lens aldose reductase activation induced by UV-irradiation under relatively high D-fructose conditions. This phenomenon can be induced by the generation of active oxygen species, and may result from intramolecular modifications of the enzyme at thiol groups.
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Mizoguchi, T., Ogura, T., Yagi, K., Kador, P.F. (1996). D-Fructose-Mediated Stimulation of Bovine Lens Aldose Reductase Activation by UV-Irradiation. In: Weiner, H., Lindahl, R., Crabb, D.W., Flynn, T.G. (eds) Enzymology and Molecular Biology of Carbonyl Metabolism 6. Advances in Experimental Medicine and Biology, vol 414. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5871-2_60
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DOI: https://doi.org/10.1007/978-1-4615-5871-2_60
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