Abstract
Assay of purine enzymes in cells is important in determining diagnosis and prognosis in purine enzyme deficiencies. Earlier methods for determination of purine enzyme activities have relied on the use of radiolabelled substrates in conjunction with thin layer Chromatographic techniques.1 More recently HPLC measurements of enzyme activities in lysed erythrocytes were introduced, which eliminated the hazards due to isotopes, but also reduces costs and shortened the time taken to obtain a diagnosis.2
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Adam, T., Ševčík, J., Fairbanks, L.D., Barták, P. (1998). Determination of Purine Enzyme Activities in Human Erythrocytes by Capillary Electrophoresis. In: Griesmacher, A., Müller, M.M., Chiba, P. (eds) Purine and Pyrimidine Metabolism in Man IX. Advances in Experimental Medicine and Biology, vol 431. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5381-6_145
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DOI: https://doi.org/10.1007/978-1-4615-5381-6_145
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