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The yeast expression system for recombinant glycosyltransferases

  • Chapter
Glycotechnology

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Abstract

Glycosyltransferases are increasingly being used for in vitro synthesis of oligosaccharides. Since these enzymes are difficult to purify from natural sources, expression systems for soluble forms of the recombinant enzymes have been developed. This review focuses on the current state of development of yeast expression systems. Two yeast species have mainly been used, i.e. Saccharomyces cerevisiae and Pichia pastoris. Safety and ease of fermentation are well recognized for S. cerevisiae as a biotechnological expression system; however, even soluble forms of recombinant glycosyltransferases are not secreted. In some cases, hyperglycosylation may occur. P. pastoris,by contrast, secrete soluble orthoglycosylated forms to the supernatant where they can be recovered in a highly purified form.

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Authors and Affiliations

  1. Institute of Physiology, University Zurich, Winterthurerstr. 190, Zürich, CH-8057, Switzerland

    Martine Malissard, Steffen Zeng & Eric G. Berger

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  1. Martine Malissard
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  2. Steffen Zeng
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  3. Eric G. Berger
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Editors and Affiliations

  1. Department of Physiology, University of Zurich, Switzerland

    E. G. Berger

  2. Department of Oral Diagnostics, University of Copenhagen, Denmark

    H. Clausen

  3. Department of Biochemistry & Molecular Biology, University of Oklahoma, USA

    R. D. Cummings

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Malissard, M., Zeng, S., Berger, E.G. (1999). The yeast expression system for recombinant glycosyltransferases. In: Berger, E.G., Clausen, H., Cummings, R.D. (eds) Glycotechnology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5257-4_4

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