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Genome Modification by Triplex-Forming Oligonucleotides

  • Chapter
Triple Helix Forming Oligonucleotides

Part of the book series: Perspectives in Antisense Science ((DARE,volume 2))

Abstract

Gene expression is a process that is first regulated at the level of DNA. By introducing modifications in specific DNA sequences of living cells, it is possible to permanently alter the expression of genes relevant to disease. Triplex technology offers an approach to site-specific genome modification in mammalian cells such that it is possible to direct damage to specific sites in the DNA via triplex-forming oligonucleotides (TFOs) and thereby induce mutations or sensitize a site for gene replacement via homologous recombination [also reviewed in (1, 2)]. The work reported in this chapter describes the initial steps toward the development of a triplex-based strategy for site-specific genome modification via targeted mutagenesis and recombination.

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Authors
  1. Karen M. Vasquez
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  2. Peter M. Glazer
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Editor information

Editors and Affiliations

  1. CNRS, France

    Claude Malvy , Annick Harel-Bellan  & Linda L. Pritchard ,  & 

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© 1999 Springer Science+Business Media New York

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Vasquez, K.M., Glazer, P.M. (1999). Genome Modification by Triplex-Forming Oligonucleotides. In: Malvy, C., Harel-Bellan, A., Pritchard, L.L. (eds) Triple Helix Forming Oligonucleotides. Perspectives in Antisense Science, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5177-5_13

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  • DOI: https://doi.org/10.1007/978-1-4615-5177-5_13

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7358-2

  • Online ISBN: 978-1-4615-5177-5

  • eBook Packages: Springer Book Archive

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