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Measurement of Oxygen Diffusion Through Cultured Endothelial Cell Monolayers

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Oxygen Transport to Tissue XXI

Abstract

Accurate values for the oxygen diffusion coefficient (DO2), the oxygen solubility (αO2), and/or the oxygen permeability (PO2 = DO2 × αO2) of substances which contribute significantly to the diffusion resistance to oxygen in tissue are important for understanding oxygen diffusion from capillaries into tissue. One of the possible important resistances to the transport of oxygen in tissue is the capillary endothelial cell wall, because of the high flux of oxygen through this wall out of the capillary. About the importance of this resistance, e.g., Groebe (1996) estimates the capillary vicinity to account for about half of the total resistance to oxygen diffusion, in dog gracilis muscle.

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References

  • Bouwer ST (1987) Experimental set-up. In: Facilitated oxygen diffusion through hemoglobin solutions. Measurement of diffusion and reaction parameters. (PhD Thesis) University of Nijmegen, pp. 46-48.

    Google Scholar 

  • van Dijk AMC, Hoofd LJC, and Oeseburg B (1996) Diffusion coefficient of oxygen in various model layers as determined by analysis of time-dependent diffusion. Adv Exp Med Biol 388:327–331.

    Article  PubMed  Google Scholar 

  • Groebe K (1996) Practical applications of models of oxygen supply, diffusion, and consumption. Past, Perspectives, and Problems. Adv Exp Med Biol 388:161–175.

    Article  CAS  Google Scholar 

  • van Hinsbergh VWM (1997) Endothelial permeability for macromolecules. Mechanistic aspects of pathophys-iological modulation. Arterioscler Thromb Vasc Biol 17:1018–1023.

    Article  Google Scholar 

  • Hoofd L and Lamboo A (1985) Oxygen permeability of methemoglobin solutions soaked in millipore filters. Adv Exp Med Biol 191:565–570.

    Article  PubMed  CAS  Google Scholar 

  • Hoofd L, de Koning J, Kreuzer F, and Lamboo A (1986) Determination of permeabilities or two gases from recording the partial pressure of one gas. Pflügers Arch 407:320–326.

    Article  PubMed  CAS  Google Scholar 

  • de Koning J, Hoofd LJC, and Kreuzer F (1981) Oxygen transport and the function of myoglobin. Theoretical model and experiments in chicken gizzard smooth muscle. Pflügers Arch 389:211–217.

    Article  PubMed  Google Scholar 

  • Liu CY, Eskin SG, and Heliums JD (1994) The oxygen permeability of cultured endothelial cell monolayers. Adv Exp Med Biol 345:723–730.

    Article  PubMed  CAS  Google Scholar 

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© 1999 Springer Science+Business Media New York

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Lamers-Lemmers, J.P.W.M., Hoofd, L.J.C., Otte-Höller, I., de Waal, R.M.W., Oeseburg, B. (1999). Measurement of Oxygen Diffusion Through Cultured Endothelial Cell Monolayers. In: Eke, A., Delpy, D.T. (eds) Oxygen Transport to Tissue XXI. Advances in Experimental Medicine and Biology, vol 471. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4717-4_79

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  • DOI: https://doi.org/10.1007/978-1-4615-4717-4_79

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7137-3

  • Online ISBN: 978-1-4615-4717-4

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