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Rice Genome Research: An Alternative Approach Based on Molecular Cytology

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Genomes

Part of the book series: Stadler Genetics Symposia Series ((SGSS))

Abstract

A genome is a collection of chromosomes and visible chromosomes under a photomicroscope that provide a unique and effective approach to genome analysis. In situ hybridization (ISH) is one of the most effective methods to analyze the essential characteristics of genomes, because the method is based on the structural constituent of genomes that is sometimes overlooked by biochemical means. The method requires that the structure of DNA fibers, chromosomes and nuclei is maintained as much as possible in order to utilize the morphological components as the key information for specifying and/or interpreting the location and biological significance of the signals detected. It could also be pointed out that the information from single chromosomal spreads sometimes gives satisfactory and essential information on the localization of certain nucleotide sequences on the target chromosomes and their biological significance. Therefore, genome analysis by ISH provides unique information different from the ordinary biochemical means which handle the mass of DNA fragments as the target in most cases.

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Authors and Affiliations

  1. Department of Biotechnology Graduate School of Engineering, Osaka University, Suita, 565-0871, Japan

    Kiichi Fukui

  2. Laboratory of Rice Genetic Engineering, Hokuriku National Agricultural Experiment Station, Joetsu, 943-0193, Japan

    Nobuko Ohmido

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  1. Kiichi Fukui
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  2. Nobuko Ohmido
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  1. University of Missouri, Columbia, Missouri, USA

    J. Perry Gustafson

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Fukui, K., Ohmido, N. (2000). Rice Genome Research: An Alternative Approach Based on Molecular Cytology. In: Gustafson, J.P. (eds) Genomes. Stadler Genetics Symposia Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4235-3_10

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  • DOI: https://doi.org/10.1007/978-1-4615-4235-3_10

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