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Elucidation of Biosynthetic Pathways by Retrodictive/Predictive Comparison of Isotopomer Patterns Determined by NMR Spectroscopy

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Genetic Engineering

Part of the book series: Genetic Engineering ((GEPM,volume 22))

Abstract

Isotopes have been crucial for the elucidation of numerous metabolic pathways such as the photosynthetic CO2 fixation cycle of plants, for which the Nobel Prize was awarded to Melvin Calvin. On the other hand, it is important to note that the biosynthetic literature is replete with incorrect conclusions derived from isotopic studies. To give just one example, numerous plant terpenes have been claimed incorrectly to be biosynthesized via the mevalonate pathway on the basis of studies with radiolabeled precursors. Only recently, the biosynthesis of many plant terpenoids versus the hitherto unknown deoxyxylulose pathway has been established [for reviews see (1–3)].

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Authors and Affiliations

  1. Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstr. 4, D-85747, Garching, Germany

    Wolfgang Eisenreich & Adelbert Bacher

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  1. Wolfgang Eisenreich
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  2. Adelbert Bacher
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  1. Brookhaven National Laboratory, Upton, New York, USA

    Jane K. Setlow

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Eisenreich, W., Bacher, A. (2000). Elucidation of Biosynthetic Pathways by Retrodictive/Predictive Comparison of Isotopomer Patterns Determined by NMR Spectroscopy. In: Setlow, J.K. (eds) Genetic Engineering. Genetic Engineering, vol 22. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4199-8_8

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  • DOI: https://doi.org/10.1007/978-1-4615-4199-8_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6884-7

  • Online ISBN: 978-1-4615-4199-8

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