Summary
A monoclonal antibody resistant (MAR) variant of MHV was isolated after infection of hybridoma cells secreting the neutralizing and fusion-inhibiting monoclonal antibody, mAb 11F. The isolated variant was able to mediate syncytia formation even in the presence of high concentrations of mAb 11F. The S gene of the variant was cloned and sequenced. There were three nucleotide exchanges in comparison to the wild-type S gene, resulting in two amino acid alterations. However, both amino acid substitutions (at positions 255 and 1116) were located outside the binding site of mAb 11F.
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Lee, H.-J., Shieh, C.-K., Gorbalenya,A.E., Koonin, E.V., LaMonica, N., Tuler, J., Bagdzhadzhyan, A., and Lai, M.M.C., 1991, Virology, 180:567
Pachuk, C.J., Bredenbeek, P.J., Zoltick, P.W., Spaan, W.J.M., and Weiss,S.R., 1989,Virology, 171:141
Siddell, S.G., 1982, J. Gen. Virol., 62:259
Luytjes, W., Sturman, L.S., Bredenbeek, P.J., Charic, J., van der Zeijst, B.A.M., Horzinek,M.C., and Spaan, W.J.M., 1987,Virology, 161:479
Schmidt, I., Skinner, M., and Siddell, S., 1987, J. Gen. Virol., 68:47
Collins, A.R., Knobler, R.L., Powell, H., and Buchmeier, M.J., 1982, Virology, 119:358
Vennema, H., Heijen, L., Zijderfeld, A., Horzinek, M.C., and Spaan, W.J.M., 1990, J. Virol., 64:339
Routledge, E., Stauber, R., Pfleiderer, M., and Siddell, S.G., 1991, J. Virol., 65:254
Kumanishi, T., 1967, Jap. J. Exp. Med., 37:461
Schwarz, B., Routledge, E., and Siddell, S.G., 1990, J. Virol., 64:4784
Sanger, F., Nicklen, S. and Coulson, A.R., 1977, Proc. Natl. Acad. Sci., USA, 74: 5463
Gubler, U., and Hoffman, B.J., 1983, Gene, 25:263
Wege, H., Dörries, R., and Wege, H., 1984, J. Gen. Virol., 65:1931
Staden, R., 1982, Nucleic Acids Res., 10:4731
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer Science+Business Media New York
About this chapter
Cite this chapter
Grosse, B., Siddell, S.G. (1994). Characterization of a Monoclonal Antibody Resistant Variant of MHV. In: Laude, H., Vautherot, JF. (eds) Coronaviruses. Advances in Experimental Medicine and Biology, vol 342. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2996-5_28
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2996-5_28
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6305-7
Online ISBN: 978-1-4615-2996-5
eBook Packages: Springer Book Archive