Abstract
Dendritic cells (DC) are a minor population of large, bone marrow-derived leukocytes that are distributed ubiquitously throughout the body (1,2). DC resident in the interstitial connective tissue of non-lymphoid organs are believed to be important “passenger” leukocytes that migrate to T-dependent areas of host lymphoid tissue following organ transplantation (3). Recently, it has been suggested that the chimeric cells observed in various organs of recipients of liver or other allografts include, predominantly, cells of DC lineage. These cells may play an important role in modulation of the immunological interaction between host and donor (4,5). Since the liver is the most tolerogenic of all transplanted organs (6–8) and is accepted spontaneously across major histocompatibility complex (MHC) barriers in mice without the need for immunosuppressive therapy (8), studies on mouse liver DC may provide important clues to mechanisms underlying tolerance induction. We have isolated DC-enriched cell populations from normal mouse liver and describe herein their immunophenotype and in vitro allostimulatory activity compared with freshly-isolated liver non-parenchymal cells (NPC).
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© 1995 Springer Science+Business Media New York
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Lu, L., Woo, J., Li, Y., Rao, A.S., Thomson, A.W. (1995). Isolation of Dendritic Leukocytes from Mouse Liver. In: Banchereau, J., Schmitt, D. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 378. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1971-3_122
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DOI: https://doi.org/10.1007/978-1-4615-1971-3_122
Publisher Name: Springer, Boston, MA
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