Abstract
Environmental stresses caused by drought and extremes of temperature are main factors limiting plant growth, productivity and distribution and up to 80 % of the total crop losses are caused by such climatic factors (Boyer, 1982). Consequently, increase in plant stress tolerance could have a major impact on agricultural productivity. Genetic engineering has recently been shown to provide new approaches for plant breeding and considerable efforts has been made to design strategies for genetic engineering of stress tolerance (Thomashow, 1999; Nuotio et al., 2001). Unfortunately, developmental, structural and physiological adaptations to stresses are often based on complex mechanisms involving a number of different genes (McCue and Hanson, 1990) and therefore not amenable to genetic engineering. However, some of the responses to abiotic stress appear to be based on relatively simple metabolic traits governed by a limited number of genes.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Alia, Hayashi, H., Chen, T.H.H. and Murata, N., 1998, Transformation with a gene for choline oxidase enhances the cold tolerance of Arabidopsis during germination and early growth, Plant Cell Environ. 21:232-239.
Bechtold, N., Ellis, J. and Pelletier, G., 1993, In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants, CR Acad. Sci. Paris, Life Sci. 316: 1194-1199.
Bianchi, G., Gamba, A., Limiroli, R., Pozzi, N., Elster, R., Salamini, F. and Bartels, D., 1993, The unusual sugar composition in leaves of the resurrection plant Myrothamnus flabellifolia, Physiol Plant 87: 223-226.
Blázquez, M. A., Santos, E., Flores, C. L., Martinezzapater, J. M., Salinas, J. and Gancedo, C., 1998, Isolation and molecular characterization of the Arabidopsis Tpsl gene, encoding trehalose-6-phosphate synthase, Plant J. 13:685-689.
Bohnert, H. J., Nelson, D. E. and Jensen, R. G., 1995, Adaptations to environmental stresses, Plant Cell 7:1099-1111.
Boyer, J. S., 1982, Plant productivity and environment, Science 218: 443-448.
Cabib, E. and Leloir, L.F., 1958, The biosynthesis of trehalose phosphate, J. Biol.Chem. 231:259-275.
Colaco, C., Sen, S., Thangavelu, M., Pinder, S. and Roser, B., 1992, Extraordinary stability of enzymes dried in trehalose: simplified molecular biology, Bio/Technology 10: 1007-1011.
Crowe, J.H., Crowe, L.M. and Chapman, D., 1984, Preservation of membranes in anhydrobiotic organisms: The role of trehalose, Science 223: 701.
Crowe, J.H., Hoekstra, F.A. and Crowe, L.M.A., 1992, Anhydrobiosis, Annu. Rev. Plant Physiol. 54: 579-599.
Drennan, P. M., Smith, M. T., Goldsworthy, D. and van Staden, J., 1993, The occurrence of trehalose in the leaves of the desiccation-tolerant angiosperm Myrothamnus flabellifolius Welw, J. Plant Physiol. 142:493-496.
Elbein, A., 1974, The metabolism of alpha-alpha-trehalose, Adv. Carbohydr. Chem. 30: 227-256.
Goddijn, O. and Smeekens, S., 1998, Sensing trehalose biosynthesis in plants, Plant J. 14: 143-146.
Goddijn, O. J. M., Verwoerd, T. C., Voogd, E., Krutwagen, R. W. H. H., de Graaf, P. T. H. M., Poels, J., Vandun, K., Ponstein, A. S., Damm, B. and Pen, J., 1997, Inhibition of trehalase activity enhances trehalose accumulation in transgenic plants, Plant Physiol. 113:181-190.
Gussin, A. E. S., 1972, Does trehalose occur in Angiospermae? Phytochemistry 11: 1827-1828.
Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166: 557-580.
Hare, P. D., Cress, W. A. and Van Staden, J., 1998, Dissecting the roles of osmolyte accumulation during stress, Plant, Cell and Environ. 21: 535-553.
Hayashi, H., Mustardy, L., Deshium, P., Ida, M. and Murata, N., 1997, Transformation of Arabidopsis thaliana with the codA gene for choline oxidase - accumulation of glycine betaine and enhanced tolerance to salt and cold stress, Plant J. 12:133-142.
Hoekstra, F. A., Wolkers, W. F., Buitink, J., Golovina, E. A., Crowe, J. H. and Crowe, L. M., 1997, Membrane stabilization in the dry state, Comp. Biochem. Physiol. 117: 335-341.
Holmström, K-O., Mäntylä, E., Welin, B., Mandal, A., Palva, E. T., Tunnela, O. E. and Londesborough, J., 1996, Drought tolerance in tobacco, Nature 379: 683-684.
Kishor, P. B. K., Hong, Z., Miao, G. H., Hu, C. A. A. and Verma, D. P. S., 1995, Overexpression pf DELTA-1-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants, Plant Physiol. 108:1387-1394.
Lilius, G., Holmberg, N. and Bülow, L. (1996) Enhanced NaCl stress tolerance in transgenic tobacco expressing bacterial choline dehydrogenase. Biotechnol. 14: 177-180.
Londesborough, J. and Vuorio, O., 1991, Trehalose-6-phpsphate synthase/phosphatase complex from baker's yeast: purification of a proteolytically activated form, J. Gen. Microbiol. 137: 323-330.
McCue, K. F. and Hanson, A. D., 1990, Drought and salt tolerance: towards understanding and application, Trends Biotechnol. 8: 358-362.
Müller, J., Aeschbacher, R.A., Wingler, A., Boiler, T. and Wiemken, A, 2001, Trehalose and trehalase in Arabidopsis, Plant Physol. 125: 1086-1093.
Müller, J., Boiler, T. and Wiemken, A., 1995a, Effects of validamycin A, a potent trehalase inhibitor, and phytohormones on trehalose metabolism in roots and root nodules of soybean and cowpea, Planta 197:362-368.
Müller, J., Boiler, T. and Wiemken, A., 1995b, Trehalose and trehalase in plants: recent developments, Plant Science 112:1-9.
Nuotio, S., Heino, P. and Palva, E. T., 2001, Signal traansduction under low-temperature stress, in: Crop Responses and Adaptations in Temperature Stress, A. S. Basra, ed., Food Products Press, Binghamton, New York, pp. 151-176.
Palta, J. and Weiss, L.S., 1993, Ice formation and freezing injury: an overview on the survival mechanisms and molecular aspects of injury and cold acclimation in herbaceous plants, in: Advances in plant cold hardiness, P. H. Li and L. Christerrson, eds., CRC Press, Boca Raton, Florida, USA, pp. 143-176.
Pilon-Smits, E. A. H., Ebskamp, M. J. M, Paul, M. J., Jeuken, M. J. W., Weisbeek, P.J. and Smeekens, S. C. M., 1995, Improved performance of transgenic fructan-accumulation tobacco under drought stress, Plant Physiol. 107: 125-130.
Pilon-Smits, E. A. H., Terry, N., Sears, T., Kim, H., Zayed, A., Hwang, S., Van Dun, K., Voogd, E., Verwoerd, T. C., Krutwagen, R. W. H. H. and Goddijn, O. J. M., 1998, Trehalose-producing transgenic tobacco plants show improved growth performance under drought stress, J. Plant Physiol. 152: 525-532.
Romero, C., Bellés, J. M., Vayá, J. L., Serrano, R. and Culláñez-Macià, F. A., 1997, Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants: pleiotropic phenotypes include drought tolerance, Planta 201:293-297.
Roser, B. and Colaco, C., 1993, A sweeter way to fresher food, New Scientist 15:25-28.
Sakamoto, A., Alia and Murata, N., 1998, Metabolic engineering of rice leading to biosynthesis of glycinebetaine and tolerance to salt and cold, Plant. Mol. Biol. 38: 1011-1019.
Sakamoto, A., Valverde, R., Alia, Chen, T.H.H. and Murata, N., 2000, Transformation of Arabidopsis with the codA gene for choline oxidase enhances freezing tolerance of plants, Plant J. 22:449-453.
Serrano, R., Culiañz-Maciá, F.A. and Morena, V., 1999, Genetic engineering of salt and drought tolerance with yeast regulatory genes, Sci. Hortic. 78: 261 -269.
Singer, M. A. and Lindquist, S., 1998, Thermotolerance in Saccharomyces cerevisiae: the Yin and Yang of trehalose, Trends Biotechnol. 16: 460-468.
Smirnoff, N., 1998, Plant resistance to environmental stress, Curr. Opin. Biotechnol. 9: 214-219.
Strøm, A. R. and Kaasen, I., 1993, Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression, Mol. Microbiol. 8: 205-210.
Sukumaran, N.P. and Weiser, C.J., 1972, An excised leaflet test for evaluating potato frost tolerance, Hort. Sci. 7,467-468.
Tarczynski, M. C., Jensen, R. G. and Bohnert, H. J., 1992, Expression of a bacterial mtlD gene in transgenic tobacco leads to production and accumulation of mannitol, Proc. Natl. Acad. Sci. USA 89: 2600-2604.
Tarczynski, M. C., Jensen, R. G. and Bohnert, H. J., 1993, Stress protection of transgenic tobacco by production of the osmolyte mannitol, Science 259: 508-510.
Thomashow, M.F., 1999, Plant cold acclimation: Freezing tolerance genes and regulatory mechanisms, Annu. Rev. Plant Physiol. Plant Mol. Biol. 50,571-599.
Vogel, G., Aeschbacher, R. A., Müller, J., Boiler, T. and Wiemken, A., 1998, Trehalose-6-phophate phosphatases from Arabidopsis thaliana - identification by functional complementation of yeast TPS2 mutant, Plant J. 13: 673-683.
Vuorio, E., Kalkkinen, N. and Londesborought, J., 1993, Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from yeast Saccharomyces cerevisiae, Eur. J. Biochem. 216: 849-861.
Yancey, P. H., Clark, M. E., Hand, S. C., Bowlus, R. D. and Somero, G. N., 1982, Living with water stress:evolution of osmolyte systems, Science 217:1214-1222.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer Science+Business Media New York
About this chapter
Cite this chapter
Tamminen, I. et al. (2002). Engineering Trehalose Biosynthesis Improves Stress Tolerance in Arabidopsis. In: Li, P.H., Palva, E.T. (eds) Plant Cold Hardiness. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0711-6_18
Download citation
DOI: https://doi.org/10.1007/978-1-4615-0711-6_18
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5205-1
Online ISBN: 978-1-4615-0711-6
eBook Packages: Springer Book Archive