Abstract
This study aimed at evaluating protective effect of taurine on the down-regulated expressions of thyroid hormone receptor (TR) genes in brains of mice exposed to arsenic (As). The SPF mice were randomly divided into As exposure group, protective group, and control group. The As exposure group was administered with 4 ppm As2O3 through drinking water for 60 days. The protective group was treated with both 4 ppm As2O3 and 150 mg/kg taurine. The control group was given with drinking water alone. The gene expressions of TR in the mouse brains of the three groups were analyzed by real-time PCR. Their protein expressions were examined by Western blot and immunohistochemistry. Our results showed that the gene expression of TRβ, a very important regulator of Camk4 transcription, was down-regulated in cerebral and cerebellar tissues of mice exposed to As. The expression of TRβ1 protein in the cerebral or cerebellar tissue significantly decreased in the group exposed to As compared to the control group. However, the expressions of TRβ gene and TRβ1 protein were significantly rescued in the group coadministered with taurine as antioxidant. These results indicated that taurine may have the protective effect on the down-regulated expressions of TR in brains of mice exposed to As.
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Abbreviations
- TR:
-
Thyroid hormone receptor
- As:
-
Arsenic
- LTP:
-
Long-term potentiation
- LTD:
-
Long-term depression
- Creb:
-
cAMP response element-binding protein
- TRE:
-
Thyroid hormone-response element
References
Ahn S, Ginty DD, Linden D (1999) A late phase of cerebellar long-term depression requires activation of CaMKIV and CREB. Neuron 23:559–568
Balkan J, Dogru-Abbasoglu S, Kanbagli O, Cevikbas U, Aykac-Toker G, Uysal M (2001) Taurine has a protective role against thioacetamide induced liver cirrhosis by decreasing oxidative stress. Hum Exp Toxicol 20:251–254
Balkan J, Kanbagli O, Aykac-Toker G, Uysal M (2002) Taurine treatment reduces hepatic lipids and oxidative stress in chronically bethanol treated rats. Biol Pharm Bull 25:1231–1233
Carew TJ, Sahley CL (1986) Invertebrate learning and memory: from behavior to molecules. Annu Rev Neurosci 9:435–487
Chattopadhyay S, Bhaumik S, Nag Chaudhury A, Das Gupta S (2002a) Arsenic induced changes in growth development and apoptosis in neonatal and adult brain cells in vivo and in tissue culture. Toxicol Lett 128:73–84
Chattopadhyay S, Bhaumik S, Purkayastha M, Basu S, Nag Chaudhuri A, Das Gupta S (2002b) Apoptosis and necrosis in developing brain cells due to arsenic toxicity and protection with antioxidants. Toxicol Lett 136:65–76
Dogru-Abbasoglu S, Kanbagli O, Balkan J, Cevikbas U, Aykac-Toke G, Uysal M (2001) The protective effect of taurine against thioacetamide hepatotoxicity of rats. Hum Exp Toxicol 20:23–27
Flora SJ (1999) Arsenic-induced oxidative stress and its reversibility following combined administration of N-acetylcysteine and meso 2,3-dimercaptosuccinic acid in rats. Clin Exp Pharmacol Physiol 26:865–869
Garcia-Chavez E, Santamaria A, Diaz-Barriga F, Mandeville P, Juarez BI, Jimenez-Capdeville ME (2003) Arsenite-induced formation of hydroxyl radical in the striatum of awaking rats. Brain Res 976:82–89
Huxtable RJ (1992) Physiological action of taurine. Physiol Rev 72:101–163
Iwama K, Nakajo S, Aluchi T, Nakaya K (2001) Apoptosis induced by arsenic trioxide in leukemia U937cells is dependent on activation of p38, inactivation of ERK and the calcium dependent production of superoxide. Int J Cancer 92:518–526
Kannan GM, Tripathi N, Dube SN (2001) Toxic effects of arsenic (III) on some hematopoietic and central nervous system variables in rats and guinea pigs. J Toxicol Clin Toxicol 39(7):675–682
Mahalakshmi K, Pushpakiran G, Anuradha CV (2003) Taurine prevents acrylonitrile-induced oxidative stress in rat brain. Pol J Pharmacol 55:1037–1043
Malenka RC, Bear MF (2004) LTP and LTD: an embarrassment of riches. Neuron 44:5–21
Mozzachiodi R, Byrne JH (2009) More than synaptic plasticity: role of nonsynaptic plasticity in learning and memory. Trends Neurosci 33:17–26
Murata M, Koibuchi N, Fukuda H, Murata M, Chin WW (2000) Augmentation of thyroid hormone receptor-mediated transcription by Ca2+/calmodulin-dependent protein kinase type. Endocrinology 141(6):2275–2278
Niittynen L, Nurminen ML, Korpela R, Vapaatalo H (1999) Role of arginine, taurine and homocysteine in cardiovascular diseases. Ann Med 31:318–326
Owen GR, Brenner EA (2012) Mapping molecular memory: navigating the cellular pathways of learning. Cell Mol Neurobiol 32:919–941
Piao F, Ma N, Hiraku Y, Murata M, Oikawa S, Cheng F, Zhong L, Yamauchi T, Kawanishi S, Yokoyama K (2005) Oxidative DNA damage in the brain of mice exposed to arsenic at environmental-relevant levels in relation to neurotoxicity. J Occup Health 47:445–449
Rodríguez VM, Carrizales L, Jiménez-Capdeville ME, Dufour L, Giordano M (2001) The effects of sodium arsenite exposure on behavioral parameters in the rat. Brain Res Bull 55:301–308
Rodríguez VM, Jiménez-Capdeville ME, Giordano M (2003) The effects of arsenic exposure on the nervous system. Toxicol Lett 145:1–18
Samuel S, Kathirvel R, Jayavelu T, Chinnakkannu P (2005) Protein oxidative damage in arsenic induced rat brain: influence of DL-α-lipoic acid. Toxicol Lett 155:27–34
Shaywitz AJ, Greenberg ME (1999) CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals. Annu Rev Biochem 68:821–861
Son HY, Kim H, Kwon YH (2007) Taurine prevents oxidative damage of high glucose-induced cataractogenesis in isolated rat lenses. J Nutr Sci Vitaminol 53:324–330
Tabassum H, Rehman H, Banerjee BD, Raisuddin S, Parvez S (2006) Attenuation of tamoxifen-induced hepatotoxicity by taurine in mice. Clin Chim Acta 370:129–136
Timbrell JA, Seabra V, Watereld CJ (1995) The in vivo and in vitro protective properties of taurine. Gen Pharmacol 26:453–462
Wang Y, Li S, Piao F, Hong Y, Liu P, Zhao Y (2009) Subchronic exposure to arsenic downregulated the expression of Camk4 as an important gene related to the late phase of cerebellar LTD of mice. Neurotoxicol Teratol 31(5):318–322
Wasserman GA, Liu X, Parvez F, Ahsan H, Factor-Litvak P, van Geen A, Slavkovich V, LoIacono NJ, Cheng Z, Hussain I, Momotaj H, Graziano JH (2004) Water arsenic exposure and children’s intellectual function in Araihazar, Bangladesh. Environ Health Perspect 112:1329–1333
Wright CE, Tallan HH, Linn YY (1986) Taurine: biological update. Annu Rev Biochem 55:427–453
Zhang C, Ling B, Liu J, Wang G (1999) Effect of fluoride-arsenic exposure on the neurobehavioral development of rats offspring. Wei Sheng Yan Jiu 28:337–338
Acknowledgements
This work was supported by National Nature Science Foundation of China [No. 30571584].
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Wang, Y., Piao, F., Li, Y., Wang, X., Guang, H. (2013). Protective Effect of Taurine on Down-Regulated Expression of Thyroid Hormone Receptor Genes in Brains of Mice Exposed to Arsenic. In: El Idrissi, A., L'Amoreaux, W. (eds) Taurine 8. Advances in Experimental Medicine and Biology, vol 775. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6130-2_13
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DOI: https://doi.org/10.1007/978-1-4614-6130-2_13
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